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Evaluation of reference genes for real-time RT-PCR expression studies in the plant pathogen Pectobacterium atrosepticum
BACKGROUND: Real-time RT-PCR has become a powerful technique to monitor low-abundance mRNA expression and is a useful tool when examining bacterial gene expression inside infected host tissues. However, correct evaluation of data requires accurate and reliable normalisation against internal standard...
Autores principales: | Takle, Gunnhild W, Toth, Ian K, Brurberg, May B |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151947/ https://www.ncbi.nlm.nih.gov/pubmed/17888160 http://dx.doi.org/10.1186/1471-2229-7-50 |
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