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Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores

Dynamic attachment of microtubules to kinetochores during mitosis generates pulling force, or tension, required for the high fidelity of chromosome separation. A lack of tension activates the spindle checkpoint and delays the anaphase onset. A key step in the tension–response pathway involves the ph...

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Detalles Bibliográficos
Autores principales: Wong, Oi Kwan, Fang, Guowei
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171348/
https://www.ncbi.nlm.nih.gov/pubmed/16129782
http://dx.doi.org/10.1083/jcb.200502163
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author Wong, Oi Kwan
Fang, Guowei
author_facet Wong, Oi Kwan
Fang, Guowei
author_sort Wong, Oi Kwan
collection PubMed
description Dynamic attachment of microtubules to kinetochores during mitosis generates pulling force, or tension, required for the high fidelity of chromosome separation. A lack of tension activates the spindle checkpoint and delays the anaphase onset. A key step in the tension–response pathway involves the phosphorylation of the 3F3/2 epitope by an unknown kinase on untensed kinetochores. Using a rephosphorylation assay in Xenopus laevis extracts, we identified the kinetochore-associated Polo-like kinase Plx1 as the kinase both necessary and sufficient for this phosphorylation. Indeed, Plx1 is the physiological 3F3/2 kinase involved in checkpoint response, as immunodepletion of Plx1 from checkpoint extracts abolished the 3F3/2 signal and blocked association of xMad2, xBubR1, xNdc80, and xNuf2 with kinetochores. Interestingly, the kinetochore localization of Plx1 is under the control of the checkpoint protein xMps1, as immunodepletion of xMps1 prevents binding of Plx1 to kinetochores. Thus, Plx1 couples the tension signal to cellular responses through phosphorylating the 3F3/2 epitope and targeting structural and checkpoint proteins to kinetochores.
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spelling pubmed-21713482008-03-05 Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores Wong, Oi Kwan Fang, Guowei J Cell Biol Research Articles Dynamic attachment of microtubules to kinetochores during mitosis generates pulling force, or tension, required for the high fidelity of chromosome separation. A lack of tension activates the spindle checkpoint and delays the anaphase onset. A key step in the tension–response pathway involves the phosphorylation of the 3F3/2 epitope by an unknown kinase on untensed kinetochores. Using a rephosphorylation assay in Xenopus laevis extracts, we identified the kinetochore-associated Polo-like kinase Plx1 as the kinase both necessary and sufficient for this phosphorylation. Indeed, Plx1 is the physiological 3F3/2 kinase involved in checkpoint response, as immunodepletion of Plx1 from checkpoint extracts abolished the 3F3/2 signal and blocked association of xMad2, xBubR1, xNdc80, and xNuf2 with kinetochores. Interestingly, the kinetochore localization of Plx1 is under the control of the checkpoint protein xMps1, as immunodepletion of xMps1 prevents binding of Plx1 to kinetochores. Thus, Plx1 couples the tension signal to cellular responses through phosphorylating the 3F3/2 epitope and targeting structural and checkpoint proteins to kinetochores. The Rockefeller University Press 2005-08-29 /pmc/articles/PMC2171348/ /pubmed/16129782 http://dx.doi.org/10.1083/jcb.200502163 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Wong, Oi Kwan
Fang, Guowei
Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores
title Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores
title_full Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores
title_fullStr Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores
title_full_unstemmed Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores
title_short Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores
title_sort plx1 is the 3f3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171348/
https://www.ncbi.nlm.nih.gov/pubmed/16129782
http://dx.doi.org/10.1083/jcb.200502163
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