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An AP-1/clathrin coat plays a novel and essential role in forming the Weibel-Palade bodies of endothelial cells

Clathrin provides an external scaffold to form small 50–100-nm transport vesicles. In contrast, formation of much larger dense-cored secretory granules is driven by selective aggregation of internal cargo at the trans-Golgi network; the only known role of clathrin in dense-cored secretory granules f...

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Autores principales: Lui-Roberts, Winnie W.Y., Collinson, Lucy M., Hewlett, Lindsay J., Michaux, Grégoire, Cutler, Daniel F.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171491/
https://www.ncbi.nlm.nih.gov/pubmed/16087708
http://dx.doi.org/10.1083/jcb.200503054
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author Lui-Roberts, Winnie W.Y.
Collinson, Lucy M.
Hewlett, Lindsay J.
Michaux, Grégoire
Cutler, Daniel F.
author_facet Lui-Roberts, Winnie W.Y.
Collinson, Lucy M.
Hewlett, Lindsay J.
Michaux, Grégoire
Cutler, Daniel F.
author_sort Lui-Roberts, Winnie W.Y.
collection PubMed
description Clathrin provides an external scaffold to form small 50–100-nm transport vesicles. In contrast, formation of much larger dense-cored secretory granules is driven by selective aggregation of internal cargo at the trans-Golgi network; the only known role of clathrin in dense-cored secretory granules formation is to remove missorted proteins by small, coated vesicles during maturation of these spherical organelles. The formation of Weibel-Palade bodies (WPBs) is also cargo driven, but these are cigar-shaped organelles up to 5 μm long. We hypothesized that a cytoplasmic coat might be required to make these very different structures, and we found that new and forming WPBs are extensively, sometimes completely, coated. Overexpression of an AP-180 truncation mutant that prevents clathrin coat formation or reduced AP-1 expression by small interfering RNA both block WPB formation. We propose that, in contrast to other secretory granules, cargo aggregation alone is not sufficient to form immature WPBs and that an external scaffold that contains AP-1 and clathrin is essential.
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spelling pubmed-21714912008-03-05 An AP-1/clathrin coat plays a novel and essential role in forming the Weibel-Palade bodies of endothelial cells Lui-Roberts, Winnie W.Y. Collinson, Lucy M. Hewlett, Lindsay J. Michaux, Grégoire Cutler, Daniel F. J Cell Biol Research Articles Clathrin provides an external scaffold to form small 50–100-nm transport vesicles. In contrast, formation of much larger dense-cored secretory granules is driven by selective aggregation of internal cargo at the trans-Golgi network; the only known role of clathrin in dense-cored secretory granules formation is to remove missorted proteins by small, coated vesicles during maturation of these spherical organelles. The formation of Weibel-Palade bodies (WPBs) is also cargo driven, but these are cigar-shaped organelles up to 5 μm long. We hypothesized that a cytoplasmic coat might be required to make these very different structures, and we found that new and forming WPBs are extensively, sometimes completely, coated. Overexpression of an AP-180 truncation mutant that prevents clathrin coat formation or reduced AP-1 expression by small interfering RNA both block WPB formation. We propose that, in contrast to other secretory granules, cargo aggregation alone is not sufficient to form immature WPBs and that an external scaffold that contains AP-1 and clathrin is essential. The Rockefeller University Press 2005-08-15 /pmc/articles/PMC2171491/ /pubmed/16087708 http://dx.doi.org/10.1083/jcb.200503054 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Lui-Roberts, Winnie W.Y.
Collinson, Lucy M.
Hewlett, Lindsay J.
Michaux, Grégoire
Cutler, Daniel F.
An AP-1/clathrin coat plays a novel and essential role in forming the Weibel-Palade bodies of endothelial cells
title An AP-1/clathrin coat plays a novel and essential role in forming the Weibel-Palade bodies of endothelial cells
title_full An AP-1/clathrin coat plays a novel and essential role in forming the Weibel-Palade bodies of endothelial cells
title_fullStr An AP-1/clathrin coat plays a novel and essential role in forming the Weibel-Palade bodies of endothelial cells
title_full_unstemmed An AP-1/clathrin coat plays a novel and essential role in forming the Weibel-Palade bodies of endothelial cells
title_short An AP-1/clathrin coat plays a novel and essential role in forming the Weibel-Palade bodies of endothelial cells
title_sort ap-1/clathrin coat plays a novel and essential role in forming the weibel-palade bodies of endothelial cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171491/
https://www.ncbi.nlm.nih.gov/pubmed/16087708
http://dx.doi.org/10.1083/jcb.200503054
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