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An ECT2–centralspindlin complex regulates the localization and function of RhoA

In anaphase, the spindle dictates the site of contractile ring assembly. Assembly and ingression of the contractile ring involves activation of myosin-II and actin polymerization, which are triggered by the GTPase RhoA. In many cells, the central spindle affects division plane positioning via unknow...

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Detalles Bibliográficos
Autores principales: Yüce, Özlem, Piekny, Alisa, Glotzer, Michael
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171506/
https://www.ncbi.nlm.nih.gov/pubmed/16103226
http://dx.doi.org/10.1083/jcb.200501097
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author Yüce, Özlem
Piekny, Alisa
Glotzer, Michael
author_facet Yüce, Özlem
Piekny, Alisa
Glotzer, Michael
author_sort Yüce, Özlem
collection PubMed
description In anaphase, the spindle dictates the site of contractile ring assembly. Assembly and ingression of the contractile ring involves activation of myosin-II and actin polymerization, which are triggered by the GTPase RhoA. In many cells, the central spindle affects division plane positioning via unknown molecular mechanisms. Here, we dissect furrow formation in human cells and show that the RhoGEF ECT2 is required for cortical localization of RhoA and contractile ring assembly. ECT2 concentrates on the central spindle by binding to centralspindlin. Depletion of the centralspindlin component MKLP1 prevents central spindle localization of ECT2; however, RhoA, F-actin, and myosin still accumulate on the equatorial cell cortex. Depletion of the other centralspindlin component, CYK-4/MgcRacGAP, prevents cortical accumulation of RhoA, F-actin, and myosin. CYK-4 and ECT2 interact, and this interaction is cell cycle regulated via ECT2 phosphorylation. Thus, central spindle localization of ECT2 assists division plane positioning and the CYK-4 subunit of centralspindlin acts upstream of RhoA to promote furrow assembly.
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spelling pubmed-21715062008-03-05 An ECT2–centralspindlin complex regulates the localization and function of RhoA Yüce, Özlem Piekny, Alisa Glotzer, Michael J Cell Biol Research Articles In anaphase, the spindle dictates the site of contractile ring assembly. Assembly and ingression of the contractile ring involves activation of myosin-II and actin polymerization, which are triggered by the GTPase RhoA. In many cells, the central spindle affects division plane positioning via unknown molecular mechanisms. Here, we dissect furrow formation in human cells and show that the RhoGEF ECT2 is required for cortical localization of RhoA and contractile ring assembly. ECT2 concentrates on the central spindle by binding to centralspindlin. Depletion of the centralspindlin component MKLP1 prevents central spindle localization of ECT2; however, RhoA, F-actin, and myosin still accumulate on the equatorial cell cortex. Depletion of the other centralspindlin component, CYK-4/MgcRacGAP, prevents cortical accumulation of RhoA, F-actin, and myosin. CYK-4 and ECT2 interact, and this interaction is cell cycle regulated via ECT2 phosphorylation. Thus, central spindle localization of ECT2 assists division plane positioning and the CYK-4 subunit of centralspindlin acts upstream of RhoA to promote furrow assembly. The Rockefeller University Press 2005-08-15 /pmc/articles/PMC2171506/ /pubmed/16103226 http://dx.doi.org/10.1083/jcb.200501097 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Yüce, Özlem
Piekny, Alisa
Glotzer, Michael
An ECT2–centralspindlin complex regulates the localization and function of RhoA
title An ECT2–centralspindlin complex regulates the localization and function of RhoA
title_full An ECT2–centralspindlin complex regulates the localization and function of RhoA
title_fullStr An ECT2–centralspindlin complex regulates the localization and function of RhoA
title_full_unstemmed An ECT2–centralspindlin complex regulates the localization and function of RhoA
title_short An ECT2–centralspindlin complex regulates the localization and function of RhoA
title_sort ect2–centralspindlin complex regulates the localization and function of rhoa
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171506/
https://www.ncbi.nlm.nih.gov/pubmed/16103226
http://dx.doi.org/10.1083/jcb.200501097
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