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An ECT2–centralspindlin complex regulates the localization and function of RhoA
In anaphase, the spindle dictates the site of contractile ring assembly. Assembly and ingression of the contractile ring involves activation of myosin-II and actin polymerization, which are triggered by the GTPase RhoA. In many cells, the central spindle affects division plane positioning via unknow...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171506/ https://www.ncbi.nlm.nih.gov/pubmed/16103226 http://dx.doi.org/10.1083/jcb.200501097 |
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author | Yüce, Özlem Piekny, Alisa Glotzer, Michael |
author_facet | Yüce, Özlem Piekny, Alisa Glotzer, Michael |
author_sort | Yüce, Özlem |
collection | PubMed |
description | In anaphase, the spindle dictates the site of contractile ring assembly. Assembly and ingression of the contractile ring involves activation of myosin-II and actin polymerization, which are triggered by the GTPase RhoA. In many cells, the central spindle affects division plane positioning via unknown molecular mechanisms. Here, we dissect furrow formation in human cells and show that the RhoGEF ECT2 is required for cortical localization of RhoA and contractile ring assembly. ECT2 concentrates on the central spindle by binding to centralspindlin. Depletion of the centralspindlin component MKLP1 prevents central spindle localization of ECT2; however, RhoA, F-actin, and myosin still accumulate on the equatorial cell cortex. Depletion of the other centralspindlin component, CYK-4/MgcRacGAP, prevents cortical accumulation of RhoA, F-actin, and myosin. CYK-4 and ECT2 interact, and this interaction is cell cycle regulated via ECT2 phosphorylation. Thus, central spindle localization of ECT2 assists division plane positioning and the CYK-4 subunit of centralspindlin acts upstream of RhoA to promote furrow assembly. |
format | Text |
id | pubmed-2171506 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21715062008-03-05 An ECT2–centralspindlin complex regulates the localization and function of RhoA Yüce, Özlem Piekny, Alisa Glotzer, Michael J Cell Biol Research Articles In anaphase, the spindle dictates the site of contractile ring assembly. Assembly and ingression of the contractile ring involves activation of myosin-II and actin polymerization, which are triggered by the GTPase RhoA. In many cells, the central spindle affects division plane positioning via unknown molecular mechanisms. Here, we dissect furrow formation in human cells and show that the RhoGEF ECT2 is required for cortical localization of RhoA and contractile ring assembly. ECT2 concentrates on the central spindle by binding to centralspindlin. Depletion of the centralspindlin component MKLP1 prevents central spindle localization of ECT2; however, RhoA, F-actin, and myosin still accumulate on the equatorial cell cortex. Depletion of the other centralspindlin component, CYK-4/MgcRacGAP, prevents cortical accumulation of RhoA, F-actin, and myosin. CYK-4 and ECT2 interact, and this interaction is cell cycle regulated via ECT2 phosphorylation. Thus, central spindle localization of ECT2 assists division plane positioning and the CYK-4 subunit of centralspindlin acts upstream of RhoA to promote furrow assembly. The Rockefeller University Press 2005-08-15 /pmc/articles/PMC2171506/ /pubmed/16103226 http://dx.doi.org/10.1083/jcb.200501097 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Yüce, Özlem Piekny, Alisa Glotzer, Michael An ECT2–centralspindlin complex regulates the localization and function of RhoA |
title | An ECT2–centralspindlin complex regulates the localization and function of RhoA |
title_full | An ECT2–centralspindlin complex regulates the localization and function of RhoA |
title_fullStr | An ECT2–centralspindlin complex regulates the localization and function of RhoA |
title_full_unstemmed | An ECT2–centralspindlin complex regulates the localization and function of RhoA |
title_short | An ECT2–centralspindlin complex regulates the localization and function of RhoA |
title_sort | ect2–centralspindlin complex regulates the localization and function of rhoa |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171506/ https://www.ncbi.nlm.nih.gov/pubmed/16103226 http://dx.doi.org/10.1083/jcb.200501097 |
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