Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis

Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3–XMAP215 complex in this process by using purified proteins and Xenopus laevis egg...

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Autores principales: Kinoshita, Kazuhisa, Noetzel, Tim L., Pelletier, Laurence, Mechtler, Karl, Drechsel, David N., Schwager, Anne, Lee, Mike, Raff, Jordan W., Hyman, Anthony A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171544/
https://www.ncbi.nlm.nih.gov/pubmed/16172205
http://dx.doi.org/10.1083/jcb.200503023
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author Kinoshita, Kazuhisa
Noetzel, Tim L.
Pelletier, Laurence
Mechtler, Karl
Drechsel, David N.
Schwager, Anne
Lee, Mike
Raff, Jordan W.
Hyman, Anthony A.
author_facet Kinoshita, Kazuhisa
Noetzel, Tim L.
Pelletier, Laurence
Mechtler, Karl
Drechsel, David N.
Schwager, Anne
Lee, Mike
Raff, Jordan W.
Hyman, Anthony A.
author_sort Kinoshita, Kazuhisa
collection PubMed
description Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3–XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A–dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.
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spelling pubmed-21715442008-03-05 Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis Kinoshita, Kazuhisa Noetzel, Tim L. Pelletier, Laurence Mechtler, Karl Drechsel, David N. Schwager, Anne Lee, Mike Raff, Jordan W. Hyman, Anthony A. J Cell Biol Research Articles Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3–XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A–dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis. The Rockefeller University Press 2005-09-26 /pmc/articles/PMC2171544/ /pubmed/16172205 http://dx.doi.org/10.1083/jcb.200503023 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Kinoshita, Kazuhisa
Noetzel, Tim L.
Pelletier, Laurence
Mechtler, Karl
Drechsel, David N.
Schwager, Anne
Lee, Mike
Raff, Jordan W.
Hyman, Anthony A.
Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis
title Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis
title_full Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis
title_fullStr Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis
title_full_unstemmed Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis
title_short Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis
title_sort aurora a phosphorylation of tacc3/maskin is required for centrosome-dependent microtubule assembly in mitosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171544/
https://www.ncbi.nlm.nih.gov/pubmed/16172205
http://dx.doi.org/10.1083/jcb.200503023
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