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Endoplasmic reticulum stress signaling transmitted by ATF6 mediates apoptosis during muscle development

Although apoptosis occurs during myogenesis, its mechanism of initiation remains unknown. In a culture model, we demonstrate activation of caspase-12, the initiator of the endoplasmic reticulum (ER) stress-specific caspase cascade, during apoptosis associated with myoblast differentiation. Induction...

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Detalles Bibliográficos
Autores principales: Nakanishi, Keiko, Sudo, Tatsuhiko, Morishima, Nobuhiro
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171703/
https://www.ncbi.nlm.nih.gov/pubmed/15897261
http://dx.doi.org/10.1083/jcb.200412024
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author Nakanishi, Keiko
Sudo, Tatsuhiko
Morishima, Nobuhiro
author_facet Nakanishi, Keiko
Sudo, Tatsuhiko
Morishima, Nobuhiro
author_sort Nakanishi, Keiko
collection PubMed
description Although apoptosis occurs during myogenesis, its mechanism of initiation remains unknown. In a culture model, we demonstrate activation of caspase-12, the initiator of the endoplasmic reticulum (ER) stress-specific caspase cascade, during apoptosis associated with myoblast differentiation. Induction of ER stress-responsive proteins (BiP and CHOP) was also observed in both apoptotic and differentiating cells. ATF6, but not other ER stress sensors, was specifically activated during apoptosis in myoblasts, suggesting that partial but selective activation of ER stress signaling was sufficient for induction of apoptosis. Activation of caspase-12 was also detected in developing muscle of mouse embryos and gradually disappeared later. CHOP was also transiently induced. These results suggest that specific ER stress signaling transmitted by ATF6 leads to naturally occurring apoptosis during muscle development.
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spelling pubmed-21717032008-03-05 Endoplasmic reticulum stress signaling transmitted by ATF6 mediates apoptosis during muscle development Nakanishi, Keiko Sudo, Tatsuhiko Morishima, Nobuhiro J Cell Biol Research Articles Although apoptosis occurs during myogenesis, its mechanism of initiation remains unknown. In a culture model, we demonstrate activation of caspase-12, the initiator of the endoplasmic reticulum (ER) stress-specific caspase cascade, during apoptosis associated with myoblast differentiation. Induction of ER stress-responsive proteins (BiP and CHOP) was also observed in both apoptotic and differentiating cells. ATF6, but not other ER stress sensors, was specifically activated during apoptosis in myoblasts, suggesting that partial but selective activation of ER stress signaling was sufficient for induction of apoptosis. Activation of caspase-12 was also detected in developing muscle of mouse embryos and gradually disappeared later. CHOP was also transiently induced. These results suggest that specific ER stress signaling transmitted by ATF6 leads to naturally occurring apoptosis during muscle development. The Rockefeller University Press 2005-05-23 /pmc/articles/PMC2171703/ /pubmed/15897261 http://dx.doi.org/10.1083/jcb.200412024 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Nakanishi, Keiko
Sudo, Tatsuhiko
Morishima, Nobuhiro
Endoplasmic reticulum stress signaling transmitted by ATF6 mediates apoptosis during muscle development
title Endoplasmic reticulum stress signaling transmitted by ATF6 mediates apoptosis during muscle development
title_full Endoplasmic reticulum stress signaling transmitted by ATF6 mediates apoptosis during muscle development
title_fullStr Endoplasmic reticulum stress signaling transmitted by ATF6 mediates apoptosis during muscle development
title_full_unstemmed Endoplasmic reticulum stress signaling transmitted by ATF6 mediates apoptosis during muscle development
title_short Endoplasmic reticulum stress signaling transmitted by ATF6 mediates apoptosis during muscle development
title_sort endoplasmic reticulum stress signaling transmitted by atf6 mediates apoptosis during muscle development
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171703/
https://www.ncbi.nlm.nih.gov/pubmed/15897261
http://dx.doi.org/10.1083/jcb.200412024
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