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Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59(fyn) and to enhance neurite outgrowth
In spite of advances in understanding the role of the cellular prion protein (PrP) in neural cell interactions, the mechanisms of PrP function remain poorly characterized. We show that PrP interacts directly with the neural cell adhesion molecule (NCAM) and associates with NCAM at the neuronal cell...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171870/ https://www.ncbi.nlm.nih.gov/pubmed/15851519 http://dx.doi.org/10.1083/jcb.200409127 |
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author | Santuccione, Antonella Sytnyk, Vladimir Leshchyns'ka, Iryna Schachner, Melitta |
author_facet | Santuccione, Antonella Sytnyk, Vladimir Leshchyns'ka, Iryna Schachner, Melitta |
author_sort | Santuccione, Antonella |
collection | PubMed |
description | In spite of advances in understanding the role of the cellular prion protein (PrP) in neural cell interactions, the mechanisms of PrP function remain poorly characterized. We show that PrP interacts directly with the neural cell adhesion molecule (NCAM) and associates with NCAM at the neuronal cell surface. Both cis and trans interactions between NCAM at the neuronal surface and PrP promote recruitment of NCAM to lipid rafts and thereby regulate activation of fyn kinase, an enzyme involved in NCAM-mediated signaling. Cis and trans interactions between NCAM and PrP promote neurite outgrowth. When these interactions are disrupted in NCAM-deficient and PrP-deficient neurons or by PrP antibodies, NCAM/PrP-dependent neurite outgrowth is arrested, indicating that PrP is involved in nervous system development cooperating with NCAM as a signaling receptor. |
format | Text |
id | pubmed-2171870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21718702008-03-05 Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59(fyn) and to enhance neurite outgrowth Santuccione, Antonella Sytnyk, Vladimir Leshchyns'ka, Iryna Schachner, Melitta J Cell Biol Research Articles In spite of advances in understanding the role of the cellular prion protein (PrP) in neural cell interactions, the mechanisms of PrP function remain poorly characterized. We show that PrP interacts directly with the neural cell adhesion molecule (NCAM) and associates with NCAM at the neuronal cell surface. Both cis and trans interactions between NCAM at the neuronal surface and PrP promote recruitment of NCAM to lipid rafts and thereby regulate activation of fyn kinase, an enzyme involved in NCAM-mediated signaling. Cis and trans interactions between NCAM and PrP promote neurite outgrowth. When these interactions are disrupted in NCAM-deficient and PrP-deficient neurons or by PrP antibodies, NCAM/PrP-dependent neurite outgrowth is arrested, indicating that PrP is involved in nervous system development cooperating with NCAM as a signaling receptor. The Rockefeller University Press 2005-04-25 /pmc/articles/PMC2171870/ /pubmed/15851519 http://dx.doi.org/10.1083/jcb.200409127 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Santuccione, Antonella Sytnyk, Vladimir Leshchyns'ka, Iryna Schachner, Melitta Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59(fyn) and to enhance neurite outgrowth |
title | Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59(fyn) and to enhance neurite outgrowth |
title_full | Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59(fyn) and to enhance neurite outgrowth |
title_fullStr | Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59(fyn) and to enhance neurite outgrowth |
title_full_unstemmed | Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59(fyn) and to enhance neurite outgrowth |
title_short | Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59(fyn) and to enhance neurite outgrowth |
title_sort | prion protein recruits its neuronal receptor ncam to lipid rafts to activate p59(fyn) and to enhance neurite outgrowth |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171870/ https://www.ncbi.nlm.nih.gov/pubmed/15851519 http://dx.doi.org/10.1083/jcb.200409127 |
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