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Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments
Contractile myocytes provide a test of the hypothesis that cells sense their mechanical as well as molecular microenvironment, altering expression, organization, and/or morphology accordingly. Here, myoblasts were cultured on collagen strips attached to glass or polymer gels of varied elasticity. Su...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172122/ https://www.ncbi.nlm.nih.gov/pubmed/15364962 http://dx.doi.org/10.1083/jcb.200405004 |
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author | Engler, Adam J. Griffin, Maureen A. Sen, Shamik Bönnemann, Carsten G. Sweeney, H. Lee Discher, Dennis E. |
author_facet | Engler, Adam J. Griffin, Maureen A. Sen, Shamik Bönnemann, Carsten G. Sweeney, H. Lee Discher, Dennis E. |
author_sort | Engler, Adam J. |
collection | PubMed |
description | Contractile myocytes provide a test of the hypothesis that cells sense their mechanical as well as molecular microenvironment, altering expression, organization, and/or morphology accordingly. Here, myoblasts were cultured on collagen strips attached to glass or polymer gels of varied elasticity. Subsequent fusion into myotubes occurs independent of substrate flexibility. However, myosin/actin striations emerge later only on gels with stiffness typical of normal muscle (passive Young's modulus, E ∼12 kPa). On glass and much softer or stiffer gels, including gels emulating stiff dystrophic muscle, cells do not striate. In addition, myotubes grown on top of a compliant bottom layer of glass-attached myotubes (but not softer fibroblasts) will striate, whereas the bottom cells will only assemble stress fibers and vinculin-rich adhesions. Unlike sarcomere formation, adhesion strength increases monotonically versus substrate stiffness with strongest adhesion on glass. These findings have major implications for in vivo introduction of stem cells into diseased or damaged striated muscle of altered mechanical composition. |
format | Text |
id | pubmed-2172122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21721222008-03-05 Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments Engler, Adam J. Griffin, Maureen A. Sen, Shamik Bönnemann, Carsten G. Sweeney, H. Lee Discher, Dennis E. J Cell Biol Research Articles Contractile myocytes provide a test of the hypothesis that cells sense their mechanical as well as molecular microenvironment, altering expression, organization, and/or morphology accordingly. Here, myoblasts were cultured on collagen strips attached to glass or polymer gels of varied elasticity. Subsequent fusion into myotubes occurs independent of substrate flexibility. However, myosin/actin striations emerge later only on gels with stiffness typical of normal muscle (passive Young's modulus, E ∼12 kPa). On glass and much softer or stiffer gels, including gels emulating stiff dystrophic muscle, cells do not striate. In addition, myotubes grown on top of a compliant bottom layer of glass-attached myotubes (but not softer fibroblasts) will striate, whereas the bottom cells will only assemble stress fibers and vinculin-rich adhesions. Unlike sarcomere formation, adhesion strength increases monotonically versus substrate stiffness with strongest adhesion on glass. These findings have major implications for in vivo introduction of stem cells into diseased or damaged striated muscle of altered mechanical composition. The Rockefeller University Press 2004-09-13 /pmc/articles/PMC2172122/ /pubmed/15364962 http://dx.doi.org/10.1083/jcb.200405004 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Engler, Adam J. Griffin, Maureen A. Sen, Shamik Bönnemann, Carsten G. Sweeney, H. Lee Discher, Dennis E. Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments |
title | Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments |
title_full | Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments |
title_fullStr | Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments |
title_full_unstemmed | Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments |
title_short | Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments |
title_sort | myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172122/ https://www.ncbi.nlm.nih.gov/pubmed/15364962 http://dx.doi.org/10.1083/jcb.200405004 |
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