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The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK

Although the microtubule-depolymerizing KinI motor Kif2a is abundantly expressed in neuronal cells, we now show it localizes to centrosomes and spindle poles during mitosis in cultured cells. RNAi-induced knockdown of Kif2a expression inhibited cell cycle progression because cells assembled monopola...

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Autores principales: Ganem, Neil J., Compton, Duane A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172212/
https://www.ncbi.nlm.nih.gov/pubmed/15302853
http://dx.doi.org/10.1083/jcb.200404012
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author Ganem, Neil J.
Compton, Duane A.
author_facet Ganem, Neil J.
Compton, Duane A.
author_sort Ganem, Neil J.
collection PubMed
description Although the microtubule-depolymerizing KinI motor Kif2a is abundantly expressed in neuronal cells, we now show it localizes to centrosomes and spindle poles during mitosis in cultured cells. RNAi-induced knockdown of Kif2a expression inhibited cell cycle progression because cells assembled monopolar spindles. Bipolar spindle assembly was restored in cells lacking Kif2a by treatments that altered microtubule assembly (nocodazole), eliminated kinetochore–microtubule attachment (loss of Nuf2), or stabilized microtubule plus ends at kinetochores (loss of MCAK). Thus, two KinI motors, MCAK and Kif2a, play distinct roles in mitosis, and MCAK activity at kinetochores must be balanced by Kif2a activity at poles for spindle bipolarity. These treatments failed to restore bipolarity to cells lacking the activity of the kinesin Eg5. Thus, two independent pathways contribute to spindle bipolarity, with the Eg5-dependent pathway using motor force to drive spindle bipolarity and the Kif2a-dependent pathway relying on microtubule polymer dynamics to generate force for spindle bipolarity.
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spelling pubmed-21722122008-03-05 The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK Ganem, Neil J. Compton, Duane A. J Cell Biol Research Articles Although the microtubule-depolymerizing KinI motor Kif2a is abundantly expressed in neuronal cells, we now show it localizes to centrosomes and spindle poles during mitosis in cultured cells. RNAi-induced knockdown of Kif2a expression inhibited cell cycle progression because cells assembled monopolar spindles. Bipolar spindle assembly was restored in cells lacking Kif2a by treatments that altered microtubule assembly (nocodazole), eliminated kinetochore–microtubule attachment (loss of Nuf2), or stabilized microtubule plus ends at kinetochores (loss of MCAK). Thus, two KinI motors, MCAK and Kif2a, play distinct roles in mitosis, and MCAK activity at kinetochores must be balanced by Kif2a activity at poles for spindle bipolarity. These treatments failed to restore bipolarity to cells lacking the activity of the kinesin Eg5. Thus, two independent pathways contribute to spindle bipolarity, with the Eg5-dependent pathway using motor force to drive spindle bipolarity and the Kif2a-dependent pathway relying on microtubule polymer dynamics to generate force for spindle bipolarity. The Rockefeller University Press 2004-08-16 /pmc/articles/PMC2172212/ /pubmed/15302853 http://dx.doi.org/10.1083/jcb.200404012 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Ganem, Neil J.
Compton, Duane A.
The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK
title The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK
title_full The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK
title_fullStr The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK
title_full_unstemmed The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK
title_short The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK
title_sort kini kinesin kif2a is required for bipolar spindle assembly through a functional relationship with mcak
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172212/
https://www.ncbi.nlm.nih.gov/pubmed/15302853
http://dx.doi.org/10.1083/jcb.200404012
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