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Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation

High mobility group box 1 (HMGB1) is an abundant chromatin protein that acts as a cytokine when released in the extracellular milieu by necrotic and inflammatory cells. Here, we show that extracellular HMGB1 and its receptor for advanced glycation end products (RAGE) induce both migration and prolif...

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Autores principales: Palumbo, Roberta, Sampaolesi, Maurilio, De Marchis, Francesco, Tonlorenzi, Rossana, Colombetti, Sara, Mondino, Anna, Cossu, Giulio, Bianchi, Marco E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172232/
https://www.ncbi.nlm.nih.gov/pubmed/14744997
http://dx.doi.org/10.1083/jcb.200304135
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author Palumbo, Roberta
Sampaolesi, Maurilio
De Marchis, Francesco
Tonlorenzi, Rossana
Colombetti, Sara
Mondino, Anna
Cossu, Giulio
Bianchi, Marco E.
author_facet Palumbo, Roberta
Sampaolesi, Maurilio
De Marchis, Francesco
Tonlorenzi, Rossana
Colombetti, Sara
Mondino, Anna
Cossu, Giulio
Bianchi, Marco E.
author_sort Palumbo, Roberta
collection PubMed
description High mobility group box 1 (HMGB1) is an abundant chromatin protein that acts as a cytokine when released in the extracellular milieu by necrotic and inflammatory cells. Here, we show that extracellular HMGB1 and its receptor for advanced glycation end products (RAGE) induce both migration and proliferation of vessel-associated stem cells (mesoangioblasts), and thus may play a role in muscle tissue regeneration. In vitro, HMGB1 induces migration and proliferation of both adult and embryonic mesoangioblasts, and disrupts the barrier function of endothelial monolayers. In living mice, mesoangioblasts injected into the femoral artery migrate close to HMGB1-loaded heparin-Sepharose beads implanted in healthy muscle, but are unresponsive to control beads. Interestingly, α-sarcoglycan null dystrophic muscle contains elevated levels of HMGB1; however, mesoangioblasts migrate into dystrophic muscle even if their RAGE receptor is disabled. This implies that the HMGB1–RAGE interaction is sufficient, but not necessary, for mesoangioblast homing; a different pathway might coexist. Although the role of endogenous HMGB1 in the reconstruction of dystrophic muscle remains to be clarified, injected HMGB1 may be used to promote tissue regeneration.
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spelling pubmed-21722322008-03-05 Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation Palumbo, Roberta Sampaolesi, Maurilio De Marchis, Francesco Tonlorenzi, Rossana Colombetti, Sara Mondino, Anna Cossu, Giulio Bianchi, Marco E. J Cell Biol Article High mobility group box 1 (HMGB1) is an abundant chromatin protein that acts as a cytokine when released in the extracellular milieu by necrotic and inflammatory cells. Here, we show that extracellular HMGB1 and its receptor for advanced glycation end products (RAGE) induce both migration and proliferation of vessel-associated stem cells (mesoangioblasts), and thus may play a role in muscle tissue regeneration. In vitro, HMGB1 induces migration and proliferation of both adult and embryonic mesoangioblasts, and disrupts the barrier function of endothelial monolayers. In living mice, mesoangioblasts injected into the femoral artery migrate close to HMGB1-loaded heparin-Sepharose beads implanted in healthy muscle, but are unresponsive to control beads. Interestingly, α-sarcoglycan null dystrophic muscle contains elevated levels of HMGB1; however, mesoangioblasts migrate into dystrophic muscle even if their RAGE receptor is disabled. This implies that the HMGB1–RAGE interaction is sufficient, but not necessary, for mesoangioblast homing; a different pathway might coexist. Although the role of endogenous HMGB1 in the reconstruction of dystrophic muscle remains to be clarified, injected HMGB1 may be used to promote tissue regeneration. The Rockefeller University Press 2004-02-02 /pmc/articles/PMC2172232/ /pubmed/14744997 http://dx.doi.org/10.1083/jcb.200304135 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Palumbo, Roberta
Sampaolesi, Maurilio
De Marchis, Francesco
Tonlorenzi, Rossana
Colombetti, Sara
Mondino, Anna
Cossu, Giulio
Bianchi, Marco E.
Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation
title Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation
title_full Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation
title_fullStr Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation
title_full_unstemmed Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation
title_short Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation
title_sort extracellular hmgb1, a signal of tissue damage, induces mesoangioblast migration and proliferation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172232/
https://www.ncbi.nlm.nih.gov/pubmed/14744997
http://dx.doi.org/10.1083/jcb.200304135
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