In vitro FRAP reveals the ATP-dependent nuclear mobilization of the exon junction complex protein SRm160

We present a new in vitro system for characterizing the binding and mobility of enhanced green fluorescent protein (EGFP)–labeled nuclear proteins by fluorescence recovery after photobleaching in digitonin-permeabilized cells. This assay reveals that SRm160, a splicing coactivator and component of t...

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Detalles Bibliográficos
Autores principales: Wagner, Stefan, Chiosea, Simion, Ivshina, Maria, Nickerson, Jeffrey A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172284/
https://www.ncbi.nlm.nih.gov/pubmed/15024032
http://dx.doi.org/10.1083/jcb.200307002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172284/
https://www.ncbi.nlm.nih.gov/pubmed/15024032
http://dx.doi.org/10.1083/jcb.200307002

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