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In vitro FRAP reveals the ATP-dependent nuclear mobilization of the exon junction complex protein SRm160
We present a new in vitro system for characterizing the binding and mobility of enhanced green fluorescent protein (EGFP)–labeled nuclear proteins by fluorescence recovery after photobleaching in digitonin-permeabilized cells. This assay reveals that SRm160, a splicing coactivator and component of t...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
2004
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172284/ https://www.ncbi.nlm.nih.gov/pubmed/15024032 http://dx.doi.org/10.1083/jcb.200307002 |