Cargando…

A novel phosphatidylinositol(3,4,5)P(3) pathway in fission yeast

The mammalian tumor suppressor, phosphatase and tensin homologue deleted on chromosome 10 (PTEN), inhibits cell growth and survival by dephosphorylating phosphatidylinositol-(3,4,5)-trisphosphate (PI[3,4,5]P(3)). We have found a homologue of PTEN in the fission yeast, Schizosaccharomyces pombe (ptn1...

Descripción completa

Detalles Bibliográficos
Autores principales: Mitra, Prasenjit, Zhang, Yingjie, Rameh, Lucia E., Ivshina, Maria P., McCollum, Dannel, Nunnari, John J., Hendricks, Gregory M., Kerr, Monica L., Field, Seth J., Cantley, Lewis C., Ross, Alonzo H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172303/
https://www.ncbi.nlm.nih.gov/pubmed/15249580
http://dx.doi.org/10.1083/jcb.200404150
_version_ 1782145041088643072
author Mitra, Prasenjit
Zhang, Yingjie
Rameh, Lucia E.
Ivshina, Maria P.
McCollum, Dannel
Nunnari, John J.
Hendricks, Gregory M.
Kerr, Monica L.
Field, Seth J.
Cantley, Lewis C.
Ross, Alonzo H.
author_facet Mitra, Prasenjit
Zhang, Yingjie
Rameh, Lucia E.
Ivshina, Maria P.
McCollum, Dannel
Nunnari, John J.
Hendricks, Gregory M.
Kerr, Monica L.
Field, Seth J.
Cantley, Lewis C.
Ross, Alonzo H.
author_sort Mitra, Prasenjit
collection PubMed
description The mammalian tumor suppressor, phosphatase and tensin homologue deleted on chromosome 10 (PTEN), inhibits cell growth and survival by dephosphorylating phosphatidylinositol-(3,4,5)-trisphosphate (PI[3,4,5]P(3)). We have found a homologue of PTEN in the fission yeast, Schizosaccharomyces pombe (ptn1). This was an unexpected finding because yeast (S. pombe and Saccharomyces cerevisiae) lack the class I phosphoinositide 3-kinases that generate PI(3,4,5)P(3) in higher eukaryotes. Indeed, PI(3,4,5)P(3) has not been detected in yeast. Surprisingly, upon deletion of ptn1 in S. pombe, PI(3,4,5)P(3) became detectable at levels comparable to those in mammalian cells, indicating that a pathway exists for synthesis of this lipid and that the S. pombe ptn1, like mammalian PTEN, suppresses PI(3,4,5)P(3) levels. By examining various mutants, we show that synthesis of PI(3,4,5)P(3) in S. pombe requires the class III phosphoinositide 3-kinase, vps34p, and the phosphatidylinositol-4-phosphate 5-kinase, its3p, but does not require the phosphatidylinositol-3-phosphate 5-kinase, fab1p. These studies suggest that a pathway for PI(3,4,5)P(3) synthesis downstream of a class III phosphoinositide 3-kinase evolved before the appearance of class I phosphoinositide 3-kinases.
format Text
id pubmed-2172303
institution National Center for Biotechnology Information
language English
publishDate 2004
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21723032008-03-05 A novel phosphatidylinositol(3,4,5)P(3) pathway in fission yeast Mitra, Prasenjit Zhang, Yingjie Rameh, Lucia E. Ivshina, Maria P. McCollum, Dannel Nunnari, John J. Hendricks, Gregory M. Kerr, Monica L. Field, Seth J. Cantley, Lewis C. Ross, Alonzo H. J Cell Biol Research Articles The mammalian tumor suppressor, phosphatase and tensin homologue deleted on chromosome 10 (PTEN), inhibits cell growth and survival by dephosphorylating phosphatidylinositol-(3,4,5)-trisphosphate (PI[3,4,5]P(3)). We have found a homologue of PTEN in the fission yeast, Schizosaccharomyces pombe (ptn1). This was an unexpected finding because yeast (S. pombe and Saccharomyces cerevisiae) lack the class I phosphoinositide 3-kinases that generate PI(3,4,5)P(3) in higher eukaryotes. Indeed, PI(3,4,5)P(3) has not been detected in yeast. Surprisingly, upon deletion of ptn1 in S. pombe, PI(3,4,5)P(3) became detectable at levels comparable to those in mammalian cells, indicating that a pathway exists for synthesis of this lipid and that the S. pombe ptn1, like mammalian PTEN, suppresses PI(3,4,5)P(3) levels. By examining various mutants, we show that synthesis of PI(3,4,5)P(3) in S. pombe requires the class III phosphoinositide 3-kinase, vps34p, and the phosphatidylinositol-4-phosphate 5-kinase, its3p, but does not require the phosphatidylinositol-3-phosphate 5-kinase, fab1p. These studies suggest that a pathway for PI(3,4,5)P(3) synthesis downstream of a class III phosphoinositide 3-kinase evolved before the appearance of class I phosphoinositide 3-kinases. The Rockefeller University Press 2004-07-19 /pmc/articles/PMC2172303/ /pubmed/15249580 http://dx.doi.org/10.1083/jcb.200404150 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Mitra, Prasenjit
Zhang, Yingjie
Rameh, Lucia E.
Ivshina, Maria P.
McCollum, Dannel
Nunnari, John J.
Hendricks, Gregory M.
Kerr, Monica L.
Field, Seth J.
Cantley, Lewis C.
Ross, Alonzo H.
A novel phosphatidylinositol(3,4,5)P(3) pathway in fission yeast
title A novel phosphatidylinositol(3,4,5)P(3) pathway in fission yeast
title_full A novel phosphatidylinositol(3,4,5)P(3) pathway in fission yeast
title_fullStr A novel phosphatidylinositol(3,4,5)P(3) pathway in fission yeast
title_full_unstemmed A novel phosphatidylinositol(3,4,5)P(3) pathway in fission yeast
title_short A novel phosphatidylinositol(3,4,5)P(3) pathway in fission yeast
title_sort novel phosphatidylinositol(3,4,5)p(3) pathway in fission yeast
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172303/
https://www.ncbi.nlm.nih.gov/pubmed/15249580
http://dx.doi.org/10.1083/jcb.200404150
work_keys_str_mv AT mitraprasenjit anovelphosphatidylinositol345p3pathwayinfissionyeast
AT zhangyingjie anovelphosphatidylinositol345p3pathwayinfissionyeast
AT ramehluciae anovelphosphatidylinositol345p3pathwayinfissionyeast
AT ivshinamariap anovelphosphatidylinositol345p3pathwayinfissionyeast
AT mccollumdannel anovelphosphatidylinositol345p3pathwayinfissionyeast
AT nunnarijohnj anovelphosphatidylinositol345p3pathwayinfissionyeast
AT hendricksgregorym anovelphosphatidylinositol345p3pathwayinfissionyeast
AT kerrmonical anovelphosphatidylinositol345p3pathwayinfissionyeast
AT fieldsethj anovelphosphatidylinositol345p3pathwayinfissionyeast
AT cantleylewisc anovelphosphatidylinositol345p3pathwayinfissionyeast
AT rossalonzoh anovelphosphatidylinositol345p3pathwayinfissionyeast
AT mitraprasenjit novelphosphatidylinositol345p3pathwayinfissionyeast
AT zhangyingjie novelphosphatidylinositol345p3pathwayinfissionyeast
AT ramehluciae novelphosphatidylinositol345p3pathwayinfissionyeast
AT ivshinamariap novelphosphatidylinositol345p3pathwayinfissionyeast
AT mccollumdannel novelphosphatidylinositol345p3pathwayinfissionyeast
AT nunnarijohnj novelphosphatidylinositol345p3pathwayinfissionyeast
AT hendricksgregorym novelphosphatidylinositol345p3pathwayinfissionyeast
AT kerrmonical novelphosphatidylinositol345p3pathwayinfissionyeast
AT fieldsethj novelphosphatidylinositol345p3pathwayinfissionyeast
AT cantleylewisc novelphosphatidylinositol345p3pathwayinfissionyeast
AT rossalonzoh novelphosphatidylinositol345p3pathwayinfissionyeast