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Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments

E-cadherin is a key cell–cell adhesion molecule at adherens junctions (AJs) and undergoes endocytosis when AJs are disrupted by the action of extracellular signals. To elucidate the mechanism of this endocytosis, we developed here a new cell-free assay system for this reaction using the AJ-enriched...

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Autores principales: Izumi, Genkichi, Sakisaka, Toshiaki, Baba, Takeshi, Tanaka, Shintaro, Morimoto, Koji, Takai, Yoshimi
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172308/
https://www.ncbi.nlm.nih.gov/pubmed/15263019
http://dx.doi.org/10.1083/jcb.200401078
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author Izumi, Genkichi
Sakisaka, Toshiaki
Baba, Takeshi
Tanaka, Shintaro
Morimoto, Koji
Takai, Yoshimi
author_facet Izumi, Genkichi
Sakisaka, Toshiaki
Baba, Takeshi
Tanaka, Shintaro
Morimoto, Koji
Takai, Yoshimi
author_sort Izumi, Genkichi
collection PubMed
description E-cadherin is a key cell–cell adhesion molecule at adherens junctions (AJs) and undergoes endocytosis when AJs are disrupted by the action of extracellular signals. To elucidate the mechanism of this endocytosis, we developed here a new cell-free assay system for this reaction using the AJ-enriched fraction from rat liver. We found here that non-trans-interacting, but not trans-interacting, E-cadherin underwent endocytosis in a clathrin-dependent manner. The endocytosis of trans-interacting E-cadherin was inhibited by Rac and Cdc42 small G proteins, which were activated by trans-interacting E-cadherin or trans-interacting nectins, which are known to induce the formation of AJs in cooperation with E-cadherin. This inhibition was mediated by reorganization of the actin cytoskeleton by Rac and Cdc42 through IQGAP1, an actin filament-binding protein and a downstream target of Rac and Cdc42. These results indicate the important role of the Rac/Cdc42-IQGAP1 system in the dynamic organization and maintenance of the E-cadherin–based AJs.
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spelling pubmed-21723082008-03-05 Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments Izumi, Genkichi Sakisaka, Toshiaki Baba, Takeshi Tanaka, Shintaro Morimoto, Koji Takai, Yoshimi J Cell Biol Research Articles E-cadherin is a key cell–cell adhesion molecule at adherens junctions (AJs) and undergoes endocytosis when AJs are disrupted by the action of extracellular signals. To elucidate the mechanism of this endocytosis, we developed here a new cell-free assay system for this reaction using the AJ-enriched fraction from rat liver. We found here that non-trans-interacting, but not trans-interacting, E-cadherin underwent endocytosis in a clathrin-dependent manner. The endocytosis of trans-interacting E-cadherin was inhibited by Rac and Cdc42 small G proteins, which were activated by trans-interacting E-cadherin or trans-interacting nectins, which are known to induce the formation of AJs in cooperation with E-cadherin. This inhibition was mediated by reorganization of the actin cytoskeleton by Rac and Cdc42 through IQGAP1, an actin filament-binding protein and a downstream target of Rac and Cdc42. These results indicate the important role of the Rac/Cdc42-IQGAP1 system in the dynamic organization and maintenance of the E-cadherin–based AJs. The Rockefeller University Press 2004-07-19 /pmc/articles/PMC2172308/ /pubmed/15263019 http://dx.doi.org/10.1083/jcb.200401078 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Izumi, Genkichi
Sakisaka, Toshiaki
Baba, Takeshi
Tanaka, Shintaro
Morimoto, Koji
Takai, Yoshimi
Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments
title Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments
title_full Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments
title_fullStr Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments
title_full_unstemmed Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments
title_short Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments
title_sort endocytosis of e-cadherin regulated by rac and cdc42 small g proteins through iqgap1 and actin filaments
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172308/
https://www.ncbi.nlm.nih.gov/pubmed/15263019
http://dx.doi.org/10.1083/jcb.200401078
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