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Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import
Ubiquitin is a small polypeptide that is conjugated to proteins and commonly serves as a degradation signal. The attachment of ubiquitin (Ub) to a substrate proceeds through a multi-enzyme cascade involving an activating enzyme (E1), a conjugating enzyme (E2), and a protein ligase (E3). We previousl...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172591/ https://www.ncbi.nlm.nih.gov/pubmed/15545318 http://dx.doi.org/10.1083/jcb.200406001 |
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author | Plafker, Scott M. Plafker, Kendra S. Weissman, Allan M. Macara, Ian G. |
author_facet | Plafker, Scott M. Plafker, Kendra S. Weissman, Allan M. Macara, Ian G. |
author_sort | Plafker, Scott M. |
collection | PubMed |
description | Ubiquitin is a small polypeptide that is conjugated to proteins and commonly serves as a degradation signal. The attachment of ubiquitin (Ub) to a substrate proceeds through a multi-enzyme cascade involving an activating enzyme (E1), a conjugating enzyme (E2), and a protein ligase (E3). We previously demonstrated that a murine E2, UbcM2, is imported into nuclei by the transport receptor importin-11. We now show that the import mechanism for UbcM2 and two other human class III E2s (UbcH6 and UBE2E2) uniquely requires the covalent attachment of Ub to the active site cysteine of these enzymes. This coupling of E2 activation and transport arises from the selective interaction of importin-11 with the Ub-loaded forms of these enzymes. Together, these findings reveal that Ub charging can function as a nuclear import trigger, and identify a novel link between E2 regulation and karyopherin-mediated transport. |
format | Text |
id | pubmed-2172591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21725912008-03-05 Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import Plafker, Scott M. Plafker, Kendra S. Weissman, Allan M. Macara, Ian G. J Cell Biol Research Articles Ubiquitin is a small polypeptide that is conjugated to proteins and commonly serves as a degradation signal. The attachment of ubiquitin (Ub) to a substrate proceeds through a multi-enzyme cascade involving an activating enzyme (E1), a conjugating enzyme (E2), and a protein ligase (E3). We previously demonstrated that a murine E2, UbcM2, is imported into nuclei by the transport receptor importin-11. We now show that the import mechanism for UbcM2 and two other human class III E2s (UbcH6 and UBE2E2) uniquely requires the covalent attachment of Ub to the active site cysteine of these enzymes. This coupling of E2 activation and transport arises from the selective interaction of importin-11 with the Ub-loaded forms of these enzymes. Together, these findings reveal that Ub charging can function as a nuclear import trigger, and identify a novel link between E2 regulation and karyopherin-mediated transport. The Rockefeller University Press 2004-11-22 /pmc/articles/PMC2172591/ /pubmed/15545318 http://dx.doi.org/10.1083/jcb.200406001 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Plafker, Scott M. Plafker, Kendra S. Weissman, Allan M. Macara, Ian G. Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import |
title | Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import |
title_full | Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import |
title_fullStr | Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import |
title_full_unstemmed | Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import |
title_short | Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import |
title_sort | ubiquitin charging of human class iii ubiquitin-conjugating enzymes triggers their nuclear import |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172591/ https://www.ncbi.nlm.nih.gov/pubmed/15545318 http://dx.doi.org/10.1083/jcb.200406001 |
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