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Determinants of [Cl(−)] in recycling and late endosomes and Golgi complex measured using fluorescent ligands

Chloride concentration ([Cl(−)]) was measured in defined organellar compartments using fluorescently labeled transferrin, α(2)-macroglobulin, and cholera toxin B-subunit conjugated with Cl(−)-sensitive and -insensitive dyes. In pulse-chase experiments, [Cl(−)] in Tf-labeled early/recycling endosomes...

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Detalles Bibliográficos
Autores principales: Sonawane, N.D., Verkman, A.S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172765/
https://www.ncbi.nlm.nih.gov/pubmed/12668661
http://dx.doi.org/10.1083/jcb.200211098