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Determinants of [Cl(−)] in recycling and late endosomes and Golgi complex measured using fluorescent ligands
Chloride concentration ([Cl(−)]) was measured in defined organellar compartments using fluorescently labeled transferrin, α(2)-macroglobulin, and cholera toxin B-subunit conjugated with Cl(−)-sensitive and -insensitive dyes. In pulse-chase experiments, [Cl(−)] in Tf-labeled early/recycling endosomes...
Autores principales: | Sonawane, N.D., Verkman, A.S. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172765/ https://www.ncbi.nlm.nih.gov/pubmed/12668661 http://dx.doi.org/10.1083/jcb.200211098 |
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