Identification of XMAP215 as a microtubule-destabilizing factor in Xenopus egg extract by biochemical purification

Microtubules (MTs) polymerized with GMPCPP, a slowly hydrolyzable GTP analogue, are stable in buffer but are rapidly depolymerized in Xenopus egg extracts. This depolymerization is independent of three previously identified MT destabilizers (Op18, katanin, and XKCM1/KinI). We purified the factor res...

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Detalles Bibliográficos
Autores principales: Shirasu-Hiza, Mimi, Coughlin, Peg, Mitchison, Tim
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172913/
https://www.ncbi.nlm.nih.gov/pubmed/12719474
http://dx.doi.org/10.1083/jcb.200211095

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172913/
https://www.ncbi.nlm.nih.gov/pubmed/12719474
http://dx.doi.org/10.1083/jcb.200211095

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