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A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C
Signals transduced by kinases depend on the extent and duration of substrate phosphorylation. We generated genetically encoded fluorescent reporters for PKC activity that reversibly respond to stimuli activating PKC. Specifically, phosphorylation of the reporter expressed in mammalian cells causes c...
Autores principales: | Violin, Jonathan D., Zhang, Jin, Tsien, Roger Y., Newton, Alexandra C. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172956/ https://www.ncbi.nlm.nih.gov/pubmed/12782683 http://dx.doi.org/10.1083/jcb.200302125 |
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