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A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo
The RanGTP gradient across the interphase nuclear envelope and on the condensed mitotic chromosomes is essential for many cellular processes, including nucleocytoplasmic transport and spindle assembly. Although the chromosome-associated enzyme RCC1 is responsible for RanGTP production, the mechanism...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2003
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173367/ https://www.ncbi.nlm.nih.gov/pubmed/12604592 http://dx.doi.org/10.1083/jcb.200211004 |
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author | Li, Hoi Yeung Wirtz, Denis Zheng, Yixian |
author_facet | Li, Hoi Yeung Wirtz, Denis Zheng, Yixian |
author_sort | Li, Hoi Yeung |
collection | PubMed |
description | The RanGTP gradient across the interphase nuclear envelope and on the condensed mitotic chromosomes is essential for many cellular processes, including nucleocytoplasmic transport and spindle assembly. Although the chromosome-associated enzyme RCC1 is responsible for RanGTP production, the mechanism of generating and maintaining the RanGTP gradient in vivo remains unknown. Here, we report that regulator of chromosome condensation (RCC1) rapidly associates and dissociates with both interphase and mitotic chromosomes in living cells, and that this mobility is regulated during the cell cycle. Our kinetic modeling suggests that RCC1 couples its catalytic activity to chromosome binding to generate a RanGTP gradient. Indeed, we have demonstrated experimentally that the interaction of RCC1 with the chromatin is coupled to the nucleotide exchange on Ran in vivo. The coupling is due to the stable binding of the binary complex of RCC1–Ran to chromatin. Successful nucleotide exchange dissociates the binary complex, permitting the release of RCC1 and RanGTP from the chromatin and the production of RanGTP on the chromatin surface. |
format | Text |
id | pubmed-2173367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21733672008-05-01 A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo Li, Hoi Yeung Wirtz, Denis Zheng, Yixian J Cell Biol Report The RanGTP gradient across the interphase nuclear envelope and on the condensed mitotic chromosomes is essential for many cellular processes, including nucleocytoplasmic transport and spindle assembly. Although the chromosome-associated enzyme RCC1 is responsible for RanGTP production, the mechanism of generating and maintaining the RanGTP gradient in vivo remains unknown. Here, we report that regulator of chromosome condensation (RCC1) rapidly associates and dissociates with both interphase and mitotic chromosomes in living cells, and that this mobility is regulated during the cell cycle. Our kinetic modeling suggests that RCC1 couples its catalytic activity to chromosome binding to generate a RanGTP gradient. Indeed, we have demonstrated experimentally that the interaction of RCC1 with the chromatin is coupled to the nucleotide exchange on Ran in vivo. The coupling is due to the stable binding of the binary complex of RCC1–Ran to chromatin. Successful nucleotide exchange dissociates the binary complex, permitting the release of RCC1 and RanGTP from the chromatin and the production of RanGTP on the chromatin surface. The Rockefeller University Press 2003-03-03 /pmc/articles/PMC2173367/ /pubmed/12604592 http://dx.doi.org/10.1083/jcb.200211004 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Report Li, Hoi Yeung Wirtz, Denis Zheng, Yixian A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo |
title | A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo |
title_full | A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo |
title_fullStr | A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo |
title_full_unstemmed | A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo |
title_short | A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo |
title_sort | mechanism of coupling rcc1 mobility to rangtp production on the chromatin in vivo |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173367/ https://www.ncbi.nlm.nih.gov/pubmed/12604592 http://dx.doi.org/10.1083/jcb.200211004 |
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