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Direct measurement of Gag–Gag interaction during retrovirus assembly with FRET and fluorescence correlation spectroscopy

During retrovirus assembly, the polyprotein Gag directs protein multimerization, membrane binding, and RNA packaging. It is unknown whether assembly initiates through Gag–Gag interactions in the cytosol or at the plasma membrane. We used two fluorescence techniques—two-photon fluorescence resonance...

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Detalles Bibliográficos
Autores principales: Larson, Daniel R., Ma, Yu May, Vogt, Volker M., Webb, Watt W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173966/
https://www.ncbi.nlm.nih.gov/pubmed/14517204
http://dx.doi.org/10.1083/jcb.200303200