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Direct measurement of Gag–Gag interaction during retrovirus assembly with FRET and fluorescence correlation spectroscopy
During retrovirus assembly, the polyprotein Gag directs protein multimerization, membrane binding, and RNA packaging. It is unknown whether assembly initiates through Gag–Gag interactions in the cytosol or at the plasma membrane. We used two fluorescence techniques—two-photon fluorescence resonance...
Autores principales: | Larson, Daniel R., Ma, Yu May, Vogt, Volker M., Webb, Watt W. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173966/ https://www.ncbi.nlm.nih.gov/pubmed/14517204 http://dx.doi.org/10.1083/jcb.200303200 |
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