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A Cell-Free System for Regulated Exocytosis in Pc12 Cells

We have developed a cell-free system for regulated exocytosis in the PC12 neuroendocrine cell line. Secretory vesicles were preloaded with acridine orange in intact cells, and the cells were sonicated to produce flat, carrier-supported plasma membrane patches with attached vesicles. Exocytosis resul...

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Detalles Bibliográficos
Autores principales: Avery, Julia, Ellis, Darren J., Lang, Thorsten, Holroyd, Phillip, Riedel, Dietmar, Henderson, Robert M., Edwardson, J. Michael, Jahn, Reinhard
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174285/
https://www.ncbi.nlm.nih.gov/pubmed/10648564