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A Cell-Free System for Regulated Exocytosis in Pc12 Cells
We have developed a cell-free system for regulated exocytosis in the PC12 neuroendocrine cell line. Secretory vesicles were preloaded with acridine orange in intact cells, and the cells were sonicated to produce flat, carrier-supported plasma membrane patches with attached vesicles. Exocytosis resul...
Autores principales: | Avery, Julia, Ellis, Darren J., Lang, Thorsten, Holroyd, Phillip, Riedel, Dietmar, Henderson, Robert M., Edwardson, J. Michael, Jahn, Reinhard |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2000
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174285/ https://www.ncbi.nlm.nih.gov/pubmed/10648564 |
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