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Idiotype connectance in the immune system. I. Expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate

A new cross-reactive idiotope family (CRIAD8) is described that contains subpopulations of antibodies binding to different epitopes. One subpopulation occurs naturally in normal sera from strain A mice, is found mainly on IgG2 and IgG3 subclasses, does not bind p- azobenzenearsonate (ABA)+, does not...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186990/
https://www.ncbi.nlm.nih.gov/pubmed/6403653
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collection PubMed
description A new cross-reactive idiotope family (CRIAD8) is described that contains subpopulations of antibodies binding to different epitopes. One subpopulation occurs naturally in normal sera from strain A mice, is found mainly on IgG2 and IgG3 subclasses, does not bind p- azobenzenearsonate (ABA)+, does not express CRI5Ci, and can be selectively stimulated by low doses of antiidiotype antibody (AD8). The second subpopulation is not found in normal serum, binds ABA, is found on all IgG subclasses, expresses CRI5Ci, and is selectively stimulated by ABA-conjugated proteins. Since CRIAD8 was found on both subpopulations of antibody, and since each subpopulation could be selectively expanded, it was possible to study the effect that expansion of the ABA- CRIAD8+ set had on subsequent responses elicited by ABA-keyhole limpet hemocyanin (KLH) in the ABA+ CRIAD8+ set. In these experiments, prior immunization with AD8 restricted the subsequent response of the ABA+ CRIAD8+ set to ABA-KLH. Furthermore, only those doses of AD8 that stimulated the ABA-CRIAD8+ set reduced the responsiveness of the ABA+ CRIAD8+ set to ABA-KLH, suggesting that the two phenomena are causally related. These findings argue that CRIAD8 correlates well with a regulatory idiotope and that immune responses by lymphocyte clones that have different antigen-binding specificities can affect one another as a result of their sharing such an idiotope. These results strongly favor a network organization of the immune system.
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spelling pubmed-21869902008-04-17 Idiotype connectance in the immune system. I. Expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate J Exp Med Articles A new cross-reactive idiotope family (CRIAD8) is described that contains subpopulations of antibodies binding to different epitopes. One subpopulation occurs naturally in normal sera from strain A mice, is found mainly on IgG2 and IgG3 subclasses, does not bind p- azobenzenearsonate (ABA)+, does not express CRI5Ci, and can be selectively stimulated by low doses of antiidiotype antibody (AD8). The second subpopulation is not found in normal serum, binds ABA, is found on all IgG subclasses, expresses CRI5Ci, and is selectively stimulated by ABA-conjugated proteins. Since CRIAD8 was found on both subpopulations of antibody, and since each subpopulation could be selectively expanded, it was possible to study the effect that expansion of the ABA- CRIAD8+ set had on subsequent responses elicited by ABA-keyhole limpet hemocyanin (KLH) in the ABA+ CRIAD8+ set. In these experiments, prior immunization with AD8 restricted the subsequent response of the ABA+ CRIAD8+ set to ABA-KLH. Furthermore, only those doses of AD8 that stimulated the ABA-CRIAD8+ set reduced the responsiveness of the ABA+ CRIAD8+ set to ABA-KLH, suggesting that the two phenomena are causally related. These findings argue that CRIAD8 correlates well with a regulatory idiotope and that immune responses by lymphocyte clones that have different antigen-binding specificities can affect one another as a result of their sharing such an idiotope. These results strongly favor a network organization of the immune system. The Rockefeller University Press 1983-04-01 /pmc/articles/PMC2186990/ /pubmed/6403653 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Idiotype connectance in the immune system. I. Expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate
title Idiotype connectance in the immune system. I. Expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate
title_full Idiotype connectance in the immune system. I. Expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate
title_fullStr Idiotype connectance in the immune system. I. Expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate
title_full_unstemmed Idiotype connectance in the immune system. I. Expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate
title_short Idiotype connectance in the immune system. I. Expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate
title_sort idiotype connectance in the immune system. i. expression of a cross- reactive idiotype on induced anti-p-azophenylarsonate antibodies and on endogenous antibodies not specific for arsonate
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186990/
https://www.ncbi.nlm.nih.gov/pubmed/6403653