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Purification and characterization of the major iron-regulated protein expressed by pathogenic Neisseriae
This report describes a method to purify the major iron-regulated protein (MIRP) expressed by N. gonorrhoeae and N. meningitidis. This purification procedure involves maximal expression of the MIRP by growing the organisms on iron-limited media; cellular disruption by sonication followed by centrifu...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1987
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188580/ https://www.ncbi.nlm.nih.gov/pubmed/3559476 |