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Yeast two-hybrid interaction partner screening through in vivo Cre-mediated Binary Interaction Tag generation

Yeast two-hybrid (Y2H) has been successfully used for genome-wide screens to identify protein–protein interactions for several model organisms. Nonetheless, the logistics of pair-wise screening has resulted in a cumbersome and incomplete application of this method to complex genomes. Here, we develo...

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Detalles Bibliográficos
Autores principales: Hastie, Alex R., Pruitt, Steven C.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189736/
https://www.ncbi.nlm.nih.gov/pubmed/17986461
http://dx.doi.org/10.1093/nar/gkm894