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Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection
The nef gene product encoded by the mac239 proviral clone of simian immunodeficiency virus (SIV) markedly enhances viral replication and pathogenesis in vivo. We have used this biologically active nef isolate to examine the phenotype of Nef in retrovirally transduced human T cells in culture. SIV Ne...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1993
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191034/ https://www.ncbi.nlm.nih.gov/pubmed/8098729 |
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collection | PubMed |
description | The nef gene product encoded by the mac239 proviral clone of simian immunodeficiency virus (SIV) markedly enhances viral replication and pathogenesis in vivo. We have used this biologically active nef isolate to examine the phenotype of Nef in retrovirally transduced human T cells in culture. SIV Nef is shown to dramatically inhibit cell-surface expression of the CD4 glycoprotein without significantly affecting the total steady-state level of cellular CD4. This downregulation of the cell-surface CD4 receptor for human immunodeficiency virus type 1 (HIV- 1) infection correlated with the acquisition of resistance to superinfection by HIV-1. However, SIV Nef did not affect the level of gene expression directed by the HIV-1 long terminal repeat. It is hypothesized that downregulation of cell-surface CD4 by Nef facilitates the efficient release of infectious progeny virions and, hence, viral spread in vivo. |
format | Text |
id | pubmed-2191034 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21910342008-04-16 Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection J Exp Med Articles The nef gene product encoded by the mac239 proviral clone of simian immunodeficiency virus (SIV) markedly enhances viral replication and pathogenesis in vivo. We have used this biologically active nef isolate to examine the phenotype of Nef in retrovirally transduced human T cells in culture. SIV Nef is shown to dramatically inhibit cell-surface expression of the CD4 glycoprotein without significantly affecting the total steady-state level of cellular CD4. This downregulation of the cell-surface CD4 receptor for human immunodeficiency virus type 1 (HIV- 1) infection correlated with the acquisition of resistance to superinfection by HIV-1. However, SIV Nef did not affect the level of gene expression directed by the HIV-1 long terminal repeat. It is hypothesized that downregulation of cell-surface CD4 by Nef facilitates the efficient release of infectious progeny virions and, hence, viral spread in vivo. The Rockefeller University Press 1993-06-01 /pmc/articles/PMC2191034/ /pubmed/8098729 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection |
title | Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection |
title_full | Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection |
title_fullStr | Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection |
title_full_unstemmed | Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection |
title_short | Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection |
title_sort | downregulation of cell-surface cd4 expression by simian immunodeficiency virus nef prevents viral super infection |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191034/ https://www.ncbi.nlm.nih.gov/pubmed/8098729 |