Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process

BACKGROUND: Since real time PCR was first developed, several approaches to estimating the initial quantity of template in an RT-PCR reaction have been tried. While initially only the early thermal cycles corresponding to exponential duplication were used, lately there has been an effort to use all o...

Descripción completa

Detalles Bibliográficos
Autores principales: Smith, Marjo V, Miller, Chris R, Kohn, Michael, Walker, Nigel J, Portier, Chris J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194744/
https://www.ncbi.nlm.nih.gov/pubmed/17956631
http://dx.doi.org/10.1186/1471-2105-8-409

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194744/
https://www.ncbi.nlm.nih.gov/pubmed/17956631
http://dx.doi.org/10.1186/1471-2105-8-409

Ejemplares similares