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Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process
BACKGROUND: Since real time PCR was first developed, several approaches to estimating the initial quantity of template in an RT-PCR reaction have been tried. While initially only the early thermal cycles corresponding to exponential duplication were used, lately there has been an effort to use all o...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2007
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194744/ https://www.ncbi.nlm.nih.gov/pubmed/17956631 http://dx.doi.org/10.1186/1471-2105-8-409 |