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Membrane Resistance of Human Red Cells
A method has been devised to measure the specific membrane resistance of single human red cells. The cells were sucked into a 3 to 5 micron diameter pore in the end of a glass tube. By passing a small current through the cells, the total cell resistance was measured. The dimensions of the cell were...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1964
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195368/ https://www.ncbi.nlm.nih.gov/pubmed/14155431 |
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author | Johnson, S. L. Woodbury, J. W. |
author_facet | Johnson, S. L. Woodbury, J. W. |
author_sort | Johnson, S. L. |
collection | PubMed |
description | A method has been devised to measure the specific membrane resistance of single human red cells. The cells were sucked into a 3 to 5 micron diameter pore in the end of a glass tube. By passing a small current through the cells, the total cell resistance was measured. The dimensions of the cell were measured optically and the specific membrane resistance was then calculated. Leakage of current between the cell and the walls of the pore was minimized by filling this region with isotonic sucrose. The measured specific membrane resistance values of four human red cells were 6.3, 6.32, 10.0, and 19.7 ohm-cm(2). |
format | Text |
id | pubmed-2195368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1964 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21953682008-04-23 Membrane Resistance of Human Red Cells Johnson, S. L. Woodbury, J. W. J Gen Physiol Article A method has been devised to measure the specific membrane resistance of single human red cells. The cells were sucked into a 3 to 5 micron diameter pore in the end of a glass tube. By passing a small current through the cells, the total cell resistance was measured. The dimensions of the cell were measured optically and the specific membrane resistance was then calculated. Leakage of current between the cell and the walls of the pore was minimized by filling this region with isotonic sucrose. The measured specific membrane resistance values of four human red cells were 6.3, 6.32, 10.0, and 19.7 ohm-cm(2). The Rockefeller University Press 1964-05-01 /pmc/articles/PMC2195368/ /pubmed/14155431 Text en Copyright ©, 1964, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Johnson, S. L. Woodbury, J. W. Membrane Resistance of Human Red Cells |
title | Membrane Resistance of Human Red Cells |
title_full | Membrane Resistance of Human Red Cells |
title_fullStr | Membrane Resistance of Human Red Cells |
title_full_unstemmed | Membrane Resistance of Human Red Cells |
title_short | Membrane Resistance of Human Red Cells |
title_sort | membrane resistance of human red cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195368/ https://www.ncbi.nlm.nih.gov/pubmed/14155431 |
work_keys_str_mv | AT johnsonsl membraneresistanceofhumanredcells AT woodburyjw membraneresistanceofhumanredcells |