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Membrane Resistance of Human Red Cells

A method has been devised to measure the specific membrane resistance of single human red cells. The cells were sucked into a 3 to 5 micron diameter pore in the end of a glass tube. By passing a small current through the cells, the total cell resistance was measured. The dimensions of the cell were...

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Detalles Bibliográficos
Autores principales: Johnson, S. L., Woodbury, J. W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1964
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195368/
https://www.ncbi.nlm.nih.gov/pubmed/14155431
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author Johnson, S. L.
Woodbury, J. W.
author_facet Johnson, S. L.
Woodbury, J. W.
author_sort Johnson, S. L.
collection PubMed
description A method has been devised to measure the specific membrane resistance of single human red cells. The cells were sucked into a 3 to 5 micron diameter pore in the end of a glass tube. By passing a small current through the cells, the total cell resistance was measured. The dimensions of the cell were measured optically and the specific membrane resistance was then calculated. Leakage of current between the cell and the walls of the pore was minimized by filling this region with isotonic sucrose. The measured specific membrane resistance values of four human red cells were 6.3, 6.32, 10.0, and 19.7 ohm-cm(2).
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spelling pubmed-21953682008-04-23 Membrane Resistance of Human Red Cells Johnson, S. L. Woodbury, J. W. J Gen Physiol Article A method has been devised to measure the specific membrane resistance of single human red cells. The cells were sucked into a 3 to 5 micron diameter pore in the end of a glass tube. By passing a small current through the cells, the total cell resistance was measured. The dimensions of the cell were measured optically and the specific membrane resistance was then calculated. Leakage of current between the cell and the walls of the pore was minimized by filling this region with isotonic sucrose. The measured specific membrane resistance values of four human red cells were 6.3, 6.32, 10.0, and 19.7 ohm-cm(2). The Rockefeller University Press 1964-05-01 /pmc/articles/PMC2195368/ /pubmed/14155431 Text en Copyright ©, 1964, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Johnson, S. L.
Woodbury, J. W.
Membrane Resistance of Human Red Cells
title Membrane Resistance of Human Red Cells
title_full Membrane Resistance of Human Red Cells
title_fullStr Membrane Resistance of Human Red Cells
title_full_unstemmed Membrane Resistance of Human Red Cells
title_short Membrane Resistance of Human Red Cells
title_sort membrane resistance of human red cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195368/
https://www.ncbi.nlm.nih.gov/pubmed/14155431
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