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Temperature Characteristics of Excitation in Space-Clamped Squid Axons

Temperature characteristics of excitability in the squid giant axon were measured for the space-clamped axon with the double sucrose gap technique. Threshold strength-duration curves were obtained for square wave current pulses from 10 µsec to 10 msec and at temperatures from 5°C to 35°C. The thresh...

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Detalles Bibliográficos
Autor principal: Guttman, Rita
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195523/
https://www.ncbi.nlm.nih.gov/pubmed/5961351
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author Guttman, Rita
author_facet Guttman, Rita
author_sort Guttman, Rita
collection PubMed
description Temperature characteristics of excitability in the squid giant axon were measured for the space-clamped axon with the double sucrose gap technique. Threshold strength-duration curves were obtained for square wave current pulses from 10 µsec to 10 msec and at temperatures from 5°C to 35°C. The threshold change of potential, at which an action potential separated from a subthreshold response, averaged 17 mv at 20°C with a Q (10) of 1.15. The average threshold current density at rheobase was 12 µa/cm(2) at 20°C with a Q (10) of 2.35 compared to 2.3 obtained previously. At short times the threshold charge was 1.5·10(-8) coul/cm(2). This was relatively independent of temperature and occasionally showed a minimum in the temperature range. At intermediate times and all temperatures the threshold currents were less than for both the single time constant model and the two factor excitation process as developed by Hill. FitzHugh has made computer investigations of the effect of temperature on the excitation of the squid axon membrane as represented by the Hodgkin-Huxley equations. These are in general in good agreement with our experimental results.
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spelling pubmed-21955232008-04-23 Temperature Characteristics of Excitation in Space-Clamped Squid Axons Guttman, Rita J Gen Physiol Article Temperature characteristics of excitability in the squid giant axon were measured for the space-clamped axon with the double sucrose gap technique. Threshold strength-duration curves were obtained for square wave current pulses from 10 µsec to 10 msec and at temperatures from 5°C to 35°C. The threshold change of potential, at which an action potential separated from a subthreshold response, averaged 17 mv at 20°C with a Q (10) of 1.15. The average threshold current density at rheobase was 12 µa/cm(2) at 20°C with a Q (10) of 2.35 compared to 2.3 obtained previously. At short times the threshold charge was 1.5·10(-8) coul/cm(2). This was relatively independent of temperature and occasionally showed a minimum in the temperature range. At intermediate times and all temperatures the threshold currents were less than for both the single time constant model and the two factor excitation process as developed by Hill. FitzHugh has made computer investigations of the effect of temperature on the excitation of the squid axon membrane as represented by the Hodgkin-Huxley equations. These are in general in good agreement with our experimental results. The Rockefeller University Press 1966-05-01 /pmc/articles/PMC2195523/ /pubmed/5961351 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Guttman, Rita
Temperature Characteristics of Excitation in Space-Clamped Squid Axons
title Temperature Characteristics of Excitation in Space-Clamped Squid Axons
title_full Temperature Characteristics of Excitation in Space-Clamped Squid Axons
title_fullStr Temperature Characteristics of Excitation in Space-Clamped Squid Axons
title_full_unstemmed Temperature Characteristics of Excitation in Space-Clamped Squid Axons
title_short Temperature Characteristics of Excitation in Space-Clamped Squid Axons
title_sort temperature characteristics of excitation in space-clamped squid axons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195523/
https://www.ncbi.nlm.nih.gov/pubmed/5961351
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