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Analysis of Protease Activity in Live Antigen-presenting Cells Shows Regulation of the Phagosomal Proteolytic Contents During Dendritic Cell Activation
Here, we describe a new approach designed to monitor the proteolytic activity of maturing phagosomes in live antigen-presenting cells. We find that an ingested particle sequentially encounters distinct protease activities during phagosomal maturation. Incorporation of active proteases into the phago...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196045/ https://www.ncbi.nlm.nih.gov/pubmed/12186844 http://dx.doi.org/10.1084/jem.20020327 |
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author | Lennon-Duménil, Ana-Maria Bakker, Arnold H. Maehr, René Fiebiger, Edda Overkleeft, Herman S. Rosemblatt, Mario Ploegh, Hidde L. Lagaudrière-Gesbert, Cécile |
author_facet | Lennon-Duménil, Ana-Maria Bakker, Arnold H. Maehr, René Fiebiger, Edda Overkleeft, Herman S. Rosemblatt, Mario Ploegh, Hidde L. Lagaudrière-Gesbert, Cécile |
author_sort | Lennon-Duménil, Ana-Maria |
collection | PubMed |
description | Here, we describe a new approach designed to monitor the proteolytic activity of maturing phagosomes in live antigen-presenting cells. We find that an ingested particle sequentially encounters distinct protease activities during phagosomal maturation. Incorporation of active proteases into the phagosome of the macrophage cell line J774 indicates that phagosome maturation involves progressive fusion with early and late endocytic compartments. In contrast, phagosome biogenesis in bone marrow–derived dendritic cells (DCs) and macrophages preferentially involves endocytic compartments enriched in cathepsin S. Kinetics of phagosomal maturation is faster in macrophages than in DCs. Furthermore, the delivery of active proteases to the phagosome is significantly reduced after the activation of DCs with lipopolysaccharide. This observation is in agreement with the notion that DCs prevent the premature destruction of antigenic determinants to optimize T cell activation. Phagosomal maturation is therefore a tightly regulated process that varies according to the type and differentiation stage of the phagocyte. |
format | Text |
id | pubmed-2196045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21960452008-04-11 Analysis of Protease Activity in Live Antigen-presenting Cells Shows Regulation of the Phagosomal Proteolytic Contents During Dendritic Cell Activation Lennon-Duménil, Ana-Maria Bakker, Arnold H. Maehr, René Fiebiger, Edda Overkleeft, Herman S. Rosemblatt, Mario Ploegh, Hidde L. Lagaudrière-Gesbert, Cécile J Exp Med Article Here, we describe a new approach designed to monitor the proteolytic activity of maturing phagosomes in live antigen-presenting cells. We find that an ingested particle sequentially encounters distinct protease activities during phagosomal maturation. Incorporation of active proteases into the phagosome of the macrophage cell line J774 indicates that phagosome maturation involves progressive fusion with early and late endocytic compartments. In contrast, phagosome biogenesis in bone marrow–derived dendritic cells (DCs) and macrophages preferentially involves endocytic compartments enriched in cathepsin S. Kinetics of phagosomal maturation is faster in macrophages than in DCs. Furthermore, the delivery of active proteases to the phagosome is significantly reduced after the activation of DCs with lipopolysaccharide. This observation is in agreement with the notion that DCs prevent the premature destruction of antigenic determinants to optimize T cell activation. Phagosomal maturation is therefore a tightly regulated process that varies according to the type and differentiation stage of the phagocyte. The Rockefeller University Press 2002-08-19 /pmc/articles/PMC2196045/ /pubmed/12186844 http://dx.doi.org/10.1084/jem.20020327 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Lennon-Duménil, Ana-Maria Bakker, Arnold H. Maehr, René Fiebiger, Edda Overkleeft, Herman S. Rosemblatt, Mario Ploegh, Hidde L. Lagaudrière-Gesbert, Cécile Analysis of Protease Activity in Live Antigen-presenting Cells Shows Regulation of the Phagosomal Proteolytic Contents During Dendritic Cell Activation |
title | Analysis of Protease Activity in Live Antigen-presenting Cells Shows Regulation of the Phagosomal Proteolytic Contents During Dendritic Cell Activation |
title_full | Analysis of Protease Activity in Live Antigen-presenting Cells Shows Regulation of the Phagosomal Proteolytic Contents During Dendritic Cell Activation |
title_fullStr | Analysis of Protease Activity in Live Antigen-presenting Cells Shows Regulation of the Phagosomal Proteolytic Contents During Dendritic Cell Activation |
title_full_unstemmed | Analysis of Protease Activity in Live Antigen-presenting Cells Shows Regulation of the Phagosomal Proteolytic Contents During Dendritic Cell Activation |
title_short | Analysis of Protease Activity in Live Antigen-presenting Cells Shows Regulation of the Phagosomal Proteolytic Contents During Dendritic Cell Activation |
title_sort | analysis of protease activity in live antigen-presenting cells shows regulation of the phagosomal proteolytic contents during dendritic cell activation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196045/ https://www.ncbi.nlm.nih.gov/pubmed/12186844 http://dx.doi.org/10.1084/jem.20020327 |
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