Two Distinct Steps during Thymocyte Maturation from CD4(−)CD8(−) to CD4(+)CD8(+) Distinguished in the Early Growth Response (Egr)-1 Transgenic Mice with a Recombinase-activating Gene–Deficient Background

The early growth response (Egr)-1 is a zinc finger–containing transcription factor belonging to the immediate–early genes. Its expression in CD4/CD8 double negative (DN) immature thymocytes suggests that Egr-1 expression may be involved in early thymocyte development. In transgenic mice overexpressi...

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Autor principal: Miyazaki, Toru
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199048/
https://www.ncbi.nlm.nih.gov/pubmed/9294142
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author Miyazaki, Toru
author_facet Miyazaki, Toru
author_sort Miyazaki, Toru
collection PubMed
description The early growth response (Egr)-1 is a zinc finger–containing transcription factor belonging to the immediate–early genes. Its expression in CD4/CD8 double negative (DN) immature thymocytes suggests that Egr-1 expression may be involved in early thymocyte development. In transgenic mice overexpressing Egr-1 in a recombinase-activating gene–deficient background, thymocytes bypassed the block at the CD25(+)CD44(−) DN stage and matured to the immature CD8 single-positive (ISP) cell stage, but not further to the CD4/CD8 double-positive (DP) cell stage. When these mice were irradiated, thymocytes did develop to the DP stage, suggesting transcriptional induction of additional genes by irradiation that are required to promote thymocyte development from the ISP to the DP stage. These results provide genetic evidence for two distinct steps during early thymocyte development from the CD25(+)CD44(−) DN to the DP stage. The first step, from the CD25(+)CD44(−) DN to the ISP stage, can be entirely promoted by overexpression of Egr-1.
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spelling pubmed-21990482008-04-16 Two Distinct Steps during Thymocyte Maturation from CD4(−)CD8(−) to CD4(+)CD8(+) Distinguished in the Early Growth Response (Egr)-1 Transgenic Mice with a Recombinase-activating Gene–Deficient Background Miyazaki, Toru J Exp Med Article The early growth response (Egr)-1 is a zinc finger–containing transcription factor belonging to the immediate–early genes. Its expression in CD4/CD8 double negative (DN) immature thymocytes suggests that Egr-1 expression may be involved in early thymocyte development. In transgenic mice overexpressing Egr-1 in a recombinase-activating gene–deficient background, thymocytes bypassed the block at the CD25(+)CD44(−) DN stage and matured to the immature CD8 single-positive (ISP) cell stage, but not further to the CD4/CD8 double-positive (DP) cell stage. When these mice were irradiated, thymocytes did develop to the DP stage, suggesting transcriptional induction of additional genes by irradiation that are required to promote thymocyte development from the ISP to the DP stage. These results provide genetic evidence for two distinct steps during early thymocyte development from the CD25(+)CD44(−) DN to the DP stage. The first step, from the CD25(+)CD44(−) DN to the ISP stage, can be entirely promoted by overexpression of Egr-1. The Rockefeller University Press 1997-09-15 /pmc/articles/PMC2199048/ /pubmed/9294142 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Miyazaki, Toru
Two Distinct Steps during Thymocyte Maturation from CD4(−)CD8(−) to CD4(+)CD8(+) Distinguished in the Early Growth Response (Egr)-1 Transgenic Mice with a Recombinase-activating Gene–Deficient Background
title Two Distinct Steps during Thymocyte Maturation from CD4(−)CD8(−) to CD4(+)CD8(+) Distinguished in the Early Growth Response (Egr)-1 Transgenic Mice with a Recombinase-activating Gene–Deficient Background
title_full Two Distinct Steps during Thymocyte Maturation from CD4(−)CD8(−) to CD4(+)CD8(+) Distinguished in the Early Growth Response (Egr)-1 Transgenic Mice with a Recombinase-activating Gene–Deficient Background
title_fullStr Two Distinct Steps during Thymocyte Maturation from CD4(−)CD8(−) to CD4(+)CD8(+) Distinguished in the Early Growth Response (Egr)-1 Transgenic Mice with a Recombinase-activating Gene–Deficient Background
title_full_unstemmed Two Distinct Steps during Thymocyte Maturation from CD4(−)CD8(−) to CD4(+)CD8(+) Distinguished in the Early Growth Response (Egr)-1 Transgenic Mice with a Recombinase-activating Gene–Deficient Background
title_short Two Distinct Steps during Thymocyte Maturation from CD4(−)CD8(−) to CD4(+)CD8(+) Distinguished in the Early Growth Response (Egr)-1 Transgenic Mice with a Recombinase-activating Gene–Deficient Background
title_sort two distinct steps during thymocyte maturation from cd4(−)cd8(−) to cd4(+)cd8(+) distinguished in the early growth response (egr)-1 transgenic mice with a recombinase-activating gene–deficient background
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199048/
https://www.ncbi.nlm.nih.gov/pubmed/9294142
work_keys_str_mv AT miyazakitoru twodistinctstepsduringthymocytematurationfromcd4cd8tocd4cd8distinguishedintheearlygrowthresponseegr1transgenicmicewitharecombinaseactivatinggenedeficientbackground

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