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Polyploids require Bik1 for kinetochore–microtubule attachment
The attachment of kinetochores to spindle microtubules (MTs) is essential for maintaining constant ploidy in eukaryotic cells. Here, biochemical and imaging data is presented demonstrating that the budding yeast CLIP-170 orthologue Bik1is a component of the kinetochore-MT binding interface. Striking...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199317/ https://www.ncbi.nlm.nih.gov/pubmed/11756471 http://dx.doi.org/10.1083/jcb.200108119 |
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author | Lin, Haijiang de Carvalho, Pedro Kho, David Tai, Chin-Yin Pierre, Philippe Fink, Gerald R. Pellman, David |
author_facet | Lin, Haijiang de Carvalho, Pedro Kho, David Tai, Chin-Yin Pierre, Philippe Fink, Gerald R. Pellman, David |
author_sort | Lin, Haijiang |
collection | PubMed |
description | The attachment of kinetochores to spindle microtubules (MTs) is essential for maintaining constant ploidy in eukaryotic cells. Here, biochemical and imaging data is presented demonstrating that the budding yeast CLIP-170 orthologue Bik1is a component of the kinetochore-MT binding interface. Strikingly, Bik1 is not required for viability in haploid cells, but becomes essential in polyploids. The ploidy-specific requirement for BIK1 enabled us to characterize BIK1 without eliminating nonhomologous genes, providing a new approach to circumventing the overlapping function that is a common feature of the cytoskeleton. In polyploid cells, Bik1 is required before anaphase to maintain kinetochore separation and therefore contributes to the force that opposes the elastic recoil of attached sister chromatids. The role of Bik1 in kinetochore separation appears to be independent of the role of Bik1 in regulating MT dynamics. The finding that a protein involved in kinetochore–MT attachment is required for the viability of polyploids has potential implications for cancer therapeutics. |
format | Text |
id | pubmed-2199317 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21993172008-05-01 Polyploids require Bik1 for kinetochore–microtubule attachment Lin, Haijiang de Carvalho, Pedro Kho, David Tai, Chin-Yin Pierre, Philippe Fink, Gerald R. Pellman, David J Cell Biol Article The attachment of kinetochores to spindle microtubules (MTs) is essential for maintaining constant ploidy in eukaryotic cells. Here, biochemical and imaging data is presented demonstrating that the budding yeast CLIP-170 orthologue Bik1is a component of the kinetochore-MT binding interface. Strikingly, Bik1 is not required for viability in haploid cells, but becomes essential in polyploids. The ploidy-specific requirement for BIK1 enabled us to characterize BIK1 without eliminating nonhomologous genes, providing a new approach to circumventing the overlapping function that is a common feature of the cytoskeleton. In polyploid cells, Bik1 is required before anaphase to maintain kinetochore separation and therefore contributes to the force that opposes the elastic recoil of attached sister chromatids. The role of Bik1 in kinetochore separation appears to be independent of the role of Bik1 in regulating MT dynamics. The finding that a protein involved in kinetochore–MT attachment is required for the viability of polyploids has potential implications for cancer therapeutics. The Rockefeller University Press 2001-12-24 /pmc/articles/PMC2199317/ /pubmed/11756471 http://dx.doi.org/10.1083/jcb.200108119 Text en Copyright © 2001, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Lin, Haijiang de Carvalho, Pedro Kho, David Tai, Chin-Yin Pierre, Philippe Fink, Gerald R. Pellman, David Polyploids require Bik1 for kinetochore–microtubule attachment |
title | Polyploids require Bik1 for kinetochore–microtubule attachment |
title_full | Polyploids require Bik1 for kinetochore–microtubule attachment |
title_fullStr | Polyploids require Bik1 for kinetochore–microtubule attachment |
title_full_unstemmed | Polyploids require Bik1 for kinetochore–microtubule attachment |
title_short | Polyploids require Bik1 for kinetochore–microtubule attachment |
title_sort | polyploids require bik1 for kinetochore–microtubule attachment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199317/ https://www.ncbi.nlm.nih.gov/pubmed/11756471 http://dx.doi.org/10.1083/jcb.200108119 |
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