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THE ISOLATION OF MOUSE HEPATOCYTE GAP JUNCTIONS : Preliminary Chemical Characterization and X-Ray Diffraction
A method is reported for isolating a preparation of hepatic gap junctions from the mouse. The method involves a collagenase digestion, treatment with the detergent Sarkosyl NL-97, and ultrasonication, followed by sucrose gradient ultracentrifugation. A run with 36 animals yields 0.1–0.5 mg protein....
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1972
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200277/ https://www.ncbi.nlm.nih.gov/pubmed/4339819 |