Revealing biases inherent in recombination protocols

BACKGROUND: The recombination of homologous genes is an effective protein engineering tool to evolve proteins. DNA shuffling by gene fragmentation and reassembly has dominated the literature since its first publication, but this fragmentation-based method is labor intensive. Recently, a fragmentatio...

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Detalles Bibliográficos
Autores principales: Chaparro-Riggers, Javier F, Loo, Bernard LW, Polizzi, Karen M, Gibbs, Phillip R, Tang, Xiao-Song, Nelson, Mark J, Bommarius, Andreas S
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203992/
https://www.ncbi.nlm.nih.gov/pubmed/18001472
http://dx.doi.org/10.1186/1472-6750-7-77

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203992/
https://www.ncbi.nlm.nih.gov/pubmed/18001472
http://dx.doi.org/10.1186/1472-6750-7-77

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