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Lineage Diversion of T Cell Receptor Transgenic Thymocytes Revealed by Lineage Fate Mapping

BACKGROUND: The binding of the T cell receptor (TCR) to major histocompatibility complex (MHC) molecules in the thymus determines fates of TCRαβ lymphocytes that subsequently home to secondary lymphoid tissue. TCR transgenic models have been used to study thymic selection and lineage commitment. Mos...

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Autores principales: Egawa, Takeshi, Kreslavsky, Taras, Littman, Dan R., von Boehmer, Harald
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211402/
https://www.ncbi.nlm.nih.gov/pubmed/18231598
http://dx.doi.org/10.1371/journal.pone.0001512
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author Egawa, Takeshi
Kreslavsky, Taras
Littman, Dan R.
von Boehmer, Harald
author_facet Egawa, Takeshi
Kreslavsky, Taras
Littman, Dan R.
von Boehmer, Harald
author_sort Egawa, Takeshi
collection PubMed
description BACKGROUND: The binding of the T cell receptor (TCR) to major histocompatibility complex (MHC) molecules in the thymus determines fates of TCRαβ lymphocytes that subsequently home to secondary lymphoid tissue. TCR transgenic models have been used to study thymic selection and lineage commitment. Most TCR transgenic mice express the rearranged TCRαβ prematurely at the double negative stage and abnormal TCRαβ populations of T cells that are not easily detected in non-transgenic mice have been found in secondary lymphoid tissue of TCR transgenic mice. METHODOLOGY AND PRINCIPAL FINDINGS: To determine developmental pathways of TCR-transgenic thymocytes, we used Cre-LoxP-mediated fate mapping and show here that premature expression of a transgenic TCRαβ diverts some developing thymocytes to a developmental pathway which resembles that of gamma delta cells. We found that most peripheral T cells with the HY-TCR in male mice have bypassed the RORγt-positive CD4(+)8(+) (double positive, DP) stage to accumulate either as CD4(−)8(−) (double negative, DN) or as CD8α(+) T cells in lymph nodes or gut epithelium. Likewise, DN TCRαβ cells in lymphoid tissue of female mice were not derived from DP thymocytes. CONCLUSION: The results further support the hypothesis that the premature expression of the TCRαβ can divert DN thymocytes into gamma delta lineage cells.
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spelling pubmed-22114022008-01-30 Lineage Diversion of T Cell Receptor Transgenic Thymocytes Revealed by Lineage Fate Mapping Egawa, Takeshi Kreslavsky, Taras Littman, Dan R. von Boehmer, Harald PLoS One Research Article BACKGROUND: The binding of the T cell receptor (TCR) to major histocompatibility complex (MHC) molecules in the thymus determines fates of TCRαβ lymphocytes that subsequently home to secondary lymphoid tissue. TCR transgenic models have been used to study thymic selection and lineage commitment. Most TCR transgenic mice express the rearranged TCRαβ prematurely at the double negative stage and abnormal TCRαβ populations of T cells that are not easily detected in non-transgenic mice have been found in secondary lymphoid tissue of TCR transgenic mice. METHODOLOGY AND PRINCIPAL FINDINGS: To determine developmental pathways of TCR-transgenic thymocytes, we used Cre-LoxP-mediated fate mapping and show here that premature expression of a transgenic TCRαβ diverts some developing thymocytes to a developmental pathway which resembles that of gamma delta cells. We found that most peripheral T cells with the HY-TCR in male mice have bypassed the RORγt-positive CD4(+)8(+) (double positive, DP) stage to accumulate either as CD4(−)8(−) (double negative, DN) or as CD8α(+) T cells in lymph nodes or gut epithelium. Likewise, DN TCRαβ cells in lymphoid tissue of female mice were not derived from DP thymocytes. CONCLUSION: The results further support the hypothesis that the premature expression of the TCRαβ can divert DN thymocytes into gamma delta lineage cells. Public Library of Science 2008-01-30 /pmc/articles/PMC2211402/ /pubmed/18231598 http://dx.doi.org/10.1371/journal.pone.0001512 Text en Egawa et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Egawa, Takeshi
Kreslavsky, Taras
Littman, Dan R.
von Boehmer, Harald
Lineage Diversion of T Cell Receptor Transgenic Thymocytes Revealed by Lineage Fate Mapping
title Lineage Diversion of T Cell Receptor Transgenic Thymocytes Revealed by Lineage Fate Mapping
title_full Lineage Diversion of T Cell Receptor Transgenic Thymocytes Revealed by Lineage Fate Mapping
title_fullStr Lineage Diversion of T Cell Receptor Transgenic Thymocytes Revealed by Lineage Fate Mapping
title_full_unstemmed Lineage Diversion of T Cell Receptor Transgenic Thymocytes Revealed by Lineage Fate Mapping
title_short Lineage Diversion of T Cell Receptor Transgenic Thymocytes Revealed by Lineage Fate Mapping
title_sort lineage diversion of t cell receptor transgenic thymocytes revealed by lineage fate mapping
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211402/
https://www.ncbi.nlm.nih.gov/pubmed/18231598
http://dx.doi.org/10.1371/journal.pone.0001512
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