PKCβ regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1

The B cell antigen receptor (BCR)–mediated activation of IκB kinase (IKK) and nuclear factor–κB require protein kinase C (PKC)β; however, the mechanism by which PKCβ regulates IKK is unclear. Here, we demonstrate that another protein kinase, TGFβ-activated kinase (TAK)1, is essential for IKK activat...

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Autores principales: Shinohara, Hisaaki, Yasuda, Tomoharu, Aiba, Yuichi, Sanjo, Hideki, Hamadate, Megumi, Watarai, Hiroshi, Sakurai, Hiroaki, Kurosaki, Tomohiro
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212994/
https://www.ncbi.nlm.nih.gov/pubmed/16301747
http://dx.doi.org/10.1084/jem.20051591
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author Shinohara, Hisaaki
Yasuda, Tomoharu
Aiba, Yuichi
Sanjo, Hideki
Hamadate, Megumi
Watarai, Hiroshi
Sakurai, Hiroaki
Kurosaki, Tomohiro
author_facet Shinohara, Hisaaki
Yasuda, Tomoharu
Aiba, Yuichi
Sanjo, Hideki
Hamadate, Megumi
Watarai, Hiroshi
Sakurai, Hiroaki
Kurosaki, Tomohiro
author_sort Shinohara, Hisaaki
collection PubMed
description The B cell antigen receptor (BCR)–mediated activation of IκB kinase (IKK) and nuclear factor–κB require protein kinase C (PKC)β; however, the mechanism by which PKCβ regulates IKK is unclear. Here, we demonstrate that another protein kinase, TGFβ-activated kinase (TAK)1, is essential for IKK activation in response to BCR stimulation. TAK1 interacts with the phosphorylated CARMA1 (also known as caspase recruitment domain [CARD]11, Bimp3) and this interaction is mediated by PKCβ. IKK is also recruited to the CARMA1–Bcl10–mucosal-associated lymphoid tissue 1 adaptor complex in a PKCβ-dependent manner. Hence, our data suggest that phosphorylation of CARMA1, mediated by PKCβ, brings two key protein kinases, TAK1 and IKK, into close proximity, thereby allowing TAK1 to phosphorylate IKK.
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spelling pubmed-22129942008-03-11 PKCβ regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1 Shinohara, Hisaaki Yasuda, Tomoharu Aiba, Yuichi Sanjo, Hideki Hamadate, Megumi Watarai, Hiroshi Sakurai, Hiroaki Kurosaki, Tomohiro J Exp Med Article The B cell antigen receptor (BCR)–mediated activation of IκB kinase (IKK) and nuclear factor–κB require protein kinase C (PKC)β; however, the mechanism by which PKCβ regulates IKK is unclear. Here, we demonstrate that another protein kinase, TGFβ-activated kinase (TAK)1, is essential for IKK activation in response to BCR stimulation. TAK1 interacts with the phosphorylated CARMA1 (also known as caspase recruitment domain [CARD]11, Bimp3) and this interaction is mediated by PKCβ. IKK is also recruited to the CARMA1–Bcl10–mucosal-associated lymphoid tissue 1 adaptor complex in a PKCβ-dependent manner. Hence, our data suggest that phosphorylation of CARMA1, mediated by PKCβ, brings two key protein kinases, TAK1 and IKK, into close proximity, thereby allowing TAK1 to phosphorylate IKK. The Rockefeller University Press 2005-11-21 /pmc/articles/PMC2212994/ /pubmed/16301747 http://dx.doi.org/10.1084/jem.20051591 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Shinohara, Hisaaki
Yasuda, Tomoharu
Aiba, Yuichi
Sanjo, Hideki
Hamadate, Megumi
Watarai, Hiroshi
Sakurai, Hiroaki
Kurosaki, Tomohiro
PKCβ regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1
title PKCβ regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1
title_full PKCβ regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1
title_fullStr PKCβ regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1
title_full_unstemmed PKCβ regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1
title_short PKCβ regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1
title_sort pkcβ regulates bcr-mediated ikk activation by facilitating the interaction between tak1 and carma1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212994/
https://www.ncbi.nlm.nih.gov/pubmed/16301747
http://dx.doi.org/10.1084/jem.20051591
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