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Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications

BACKGROUND: EvaGreen (EG) is a newly developed DNA-binding dye that has recently been used in quantitative real-time PCR (qPCR), post-PCR DNA melt curve analysis and several other applications. However, very little is known about the physicochemical properties of the dye and their relevance to the a...

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Detalles Bibliográficos
Autores principales: Mao, Fei, Leung, Wai-Yee, Xin, Xing
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213645/
https://www.ncbi.nlm.nih.gov/pubmed/17996102
http://dx.doi.org/10.1186/1472-6750-7-76