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Expression profiling in APP23 mouse brain: inhibition of Aβ amyloidosis and inflammation in response to LXR agonist treatment
BACKGROUND: Recent studies demonstrate that in addition to its modulatory effect on APP processing, in vivo application of Liver X Receptor agonist T0901317 (T0) to APP transgenic and non-transgenic mice decreases the level of Aβ(42. )Moreover, in young Tg2576 mice T0 completely reversed contextual...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2214725/ https://www.ncbi.nlm.nih.gov/pubmed/17953774 http://dx.doi.org/10.1186/1750-1326-2-20 |
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author | Lefterov, Iliya Bookout, Angie Wang, Zhu Staufenbiel, Matthias Mangelsdorf, David Koldamova, Radosveta |
author_facet | Lefterov, Iliya Bookout, Angie Wang, Zhu Staufenbiel, Matthias Mangelsdorf, David Koldamova, Radosveta |
author_sort | Lefterov, Iliya |
collection | PubMed |
description | BACKGROUND: Recent studies demonstrate that in addition to its modulatory effect on APP processing, in vivo application of Liver X Receptor agonist T0901317 (T0) to APP transgenic and non-transgenic mice decreases the level of Aβ(42. )Moreover, in young Tg2576 mice T0 completely reversed contextual memory deficits. Compared to other tissues, the regulatory functions of LXRs in brain remain largely unexplored and our knowledge so far is limited to the cholesterol transporters and apoE. In this study we applied T0 to APP23 mice for various times and examined gene and protein expression. We also performed a series of experiments with primary brain cells derived from wild type and LXR knockout mice subjected to various LXR agonist treatments and inflammatory stimuli. RESULTS: We demonstrate an upregulation of genes related to lipid metabolism/transport, metabolism of xenobiotics and detoxification. Downregulated genes are involved in immune response and inflammation, cell death and apoptosis. Additional treatment experiments demonstrated an increase of soluble apolipoproteins E and A-I and a decrease of insoluble Aβ. In primary LXR(wt )but not in LXRα(-/-)β(-/- )microglia and astrocytes LXR agonists suppressed the inflammatory response induced by LPS or fibrillar Aβ. CONCLUSION: The results show that LXR agonists could alleviate AD pathology by acting on amyloid deposition and brain inflammation. An increased understanding of the LXR controlled regulation of Aβ aggregation and clearance systems will lead to the development of more specific and powerful agonists targeting LXR for the treatment of AD. |
format | Text |
id | pubmed-2214725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22147252008-01-26 Expression profiling in APP23 mouse brain: inhibition of Aβ amyloidosis and inflammation in response to LXR agonist treatment Lefterov, Iliya Bookout, Angie Wang, Zhu Staufenbiel, Matthias Mangelsdorf, David Koldamova, Radosveta Mol Neurodegener Research Article BACKGROUND: Recent studies demonstrate that in addition to its modulatory effect on APP processing, in vivo application of Liver X Receptor agonist T0901317 (T0) to APP transgenic and non-transgenic mice decreases the level of Aβ(42. )Moreover, in young Tg2576 mice T0 completely reversed contextual memory deficits. Compared to other tissues, the regulatory functions of LXRs in brain remain largely unexplored and our knowledge so far is limited to the cholesterol transporters and apoE. In this study we applied T0 to APP23 mice for various times and examined gene and protein expression. We also performed a series of experiments with primary brain cells derived from wild type and LXR knockout mice subjected to various LXR agonist treatments and inflammatory stimuli. RESULTS: We demonstrate an upregulation of genes related to lipid metabolism/transport, metabolism of xenobiotics and detoxification. Downregulated genes are involved in immune response and inflammation, cell death and apoptosis. Additional treatment experiments demonstrated an increase of soluble apolipoproteins E and A-I and a decrease of insoluble Aβ. In primary LXR(wt )but not in LXRα(-/-)β(-/- )microglia and astrocytes LXR agonists suppressed the inflammatory response induced by LPS or fibrillar Aβ. CONCLUSION: The results show that LXR agonists could alleviate AD pathology by acting on amyloid deposition and brain inflammation. An increased understanding of the LXR controlled regulation of Aβ aggregation and clearance systems will lead to the development of more specific and powerful agonists targeting LXR for the treatment of AD. BioMed Central 2007-10-22 /pmc/articles/PMC2214725/ /pubmed/17953774 http://dx.doi.org/10.1186/1750-1326-2-20 Text en Copyright © 2007 Lefterov et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lefterov, Iliya Bookout, Angie Wang, Zhu Staufenbiel, Matthias Mangelsdorf, David Koldamova, Radosveta Expression profiling in APP23 mouse brain: inhibition of Aβ amyloidosis and inflammation in response to LXR agonist treatment |
title | Expression profiling in APP23 mouse brain: inhibition of Aβ amyloidosis and inflammation in response to LXR agonist treatment |
title_full | Expression profiling in APP23 mouse brain: inhibition of Aβ amyloidosis and inflammation in response to LXR agonist treatment |
title_fullStr | Expression profiling in APP23 mouse brain: inhibition of Aβ amyloidosis and inflammation in response to LXR agonist treatment |
title_full_unstemmed | Expression profiling in APP23 mouse brain: inhibition of Aβ amyloidosis and inflammation in response to LXR agonist treatment |
title_short | Expression profiling in APP23 mouse brain: inhibition of Aβ amyloidosis and inflammation in response to LXR agonist treatment |
title_sort | expression profiling in app23 mouse brain: inhibition of aβ amyloidosis and inflammation in response to lxr agonist treatment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2214725/ https://www.ncbi.nlm.nih.gov/pubmed/17953774 http://dx.doi.org/10.1186/1750-1326-2-20 |
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