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Systematic analysis of mRNA 5' coding sequence incompleteness in Danio rerio: an automated EST-based approach

BACKGROUND: All standard methods for cDNA cloning are affected by a potential inability to effectively clone the 5' region of mRNA. The aim of this work was to estimate mRNA open reading frame (ORF) 5' region sequence completeness in the model organism Danio rerio (zebrafish). RESULTS: We...

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Detalles Bibliográficos
Autores principales: Frabetti, Flavia, Casadei, Raffaella, Lenzi, Luca, Canaider, Silvia, Vitale, Lorenza, Facchin, Federica, Carinci, Paolo, Zannotti, Maria, Strippoli, Pierluigi
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2222617/
https://www.ncbi.nlm.nih.gov/pubmed/18042283
http://dx.doi.org/10.1186/1745-6150-2-34