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Methods and Principles of Fixation by Freeze-Substitution
Freeze-substitution is based on rapid freezing of tissues followed by solution ("substitution") of ice at temperatures well below O°C. A 1 to 3 mm. specimen was thrown into 3:1 propane-isopentane cooled by liquid nitrogen to -175°C. (with precautions). The frozen tissue was placed in subst...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1958
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224544/ https://www.ncbi.nlm.nih.gov/pubmed/13587555 |