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Structural Changes in Mammalian Cells Associated with Cooling to -79°C
Suspensions of HeLa and S37 cells, with and without added glycerol, were cooled in stages to -79°C. and held at that temperature for 30 minutes. After warming to room temperature the cells were fixed, sectioned, and compared by phase contrast and electron microscopy with similar specimens kept at ro...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1960
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224880/ https://www.ncbi.nlm.nih.gov/pubmed/13843731 |