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THE STAINING OF THIN SECTIONS OF MOUSE PANCREAS PREPARED BY THE FERNÁNDEZ-MORÁN HELIUM II FREEZE-SUBSTITUTION METHOD
Small pieces of mouse pancreas were rapidly frozen in helium II, substituted in methanol at -75°C., and embedded in methacrylate by ultraviolet polymerization in the cold. The unstained cells show a structure similar to that after OsO(4) fixation, except that the RNP particles have little or no cont...
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| Formato: | Texto |
| Lenguaje: | English |
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The Rockefeller University Press
1960
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224966/ https://www.ncbi.nlm.nih.gov/pubmed/19866574 |