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APPLICATIONS OF FREEZE-SUBSTITUTION TO ELECTRON MICROSCOPE STUDIES OF INVERTEBRATE OOCYTES
A modified freeze-substitution process is described which gives a low percentage (less than 5 per cent) of preparations of invertebrate eggs which appear to be ice crystal-free at the resolution of the electron microscope. The mitochondria show no membranes in these preparations but can be recognize...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1961
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225041/ https://www.ncbi.nlm.nih.gov/pubmed/13740116 |