THE DIRECT ISOLATION OF THE MITOTIC APPARATUS

A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releas...

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Detalles Bibliográficos
Autores principales: Mazia, Daniel, Mitchison, J. M., Medina, Heitor, Harris, Patricia
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225108/
https://www.ncbi.nlm.nih.gov/pubmed/13768661

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225108/
https://www.ncbi.nlm.nih.gov/pubmed/13768661

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