THE DIRECT ISOLATION OF THE MITOTIC APPARATUS

A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releas...

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Detalles Bibliográficos
Autores principales: Mazia, Daniel, Mitchison, J. M., Medina, Heitor, Harris, Patricia
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225108/
https://www.ncbi.nlm.nih.gov/pubmed/13768661
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author Mazia, Daniel
Mitchison, J. M.
Medina, Heitor
Harris, Patricia
author_facet Mazia, Daniel
Mitchison, J. M.
Medina, Heitor
Harris, Patricia
author_sort Mazia, Daniel
collection PubMed
description A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.
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spelling pubmed-22251082008-05-01 THE DIRECT ISOLATION OF THE MITOTIC APPARATUS Mazia, Daniel Mitchison, J. M. Medina, Heitor Harris, Patricia J Biophys Biochem Cytol Article A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus. The Rockefeller University Press 1961-08-01 /pmc/articles/PMC2225108/ /pubmed/13768661 Text en Copyright © Copyright, 1961, by The Rockefeller Institute Press
spellingShingle Article
Mazia, Daniel
Mitchison, J. M.
Medina, Heitor
Harris, Patricia
THE DIRECT ISOLATION OF THE MITOTIC APPARATUS
title THE DIRECT ISOLATION OF THE MITOTIC APPARATUS
title_full THE DIRECT ISOLATION OF THE MITOTIC APPARATUS
title_fullStr THE DIRECT ISOLATION OF THE MITOTIC APPARATUS
title_full_unstemmed THE DIRECT ISOLATION OF THE MITOTIC APPARATUS
title_short THE DIRECT ISOLATION OF THE MITOTIC APPARATUS
title_sort direct isolation of the mitotic apparatus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225108/
https://www.ncbi.nlm.nih.gov/pubmed/13768661
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