Expression, purification and crystallization of 2-­oxo-hept-4-ene-1,7-dioate hydratase (HpcG) from Escherichia coli C

The gene encoding 2-oxo-hept-3-ene-1,7-dioic acid (OHED) hydratase (HpcG) was cloned into the high-expression plasmid pET26b and overexpressed in Escherichia coli BL21(DE3). The enzyme was purified in three steps to greater than 95% purity prior to crystallization. Crystals were obtained by the hang...

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Detalles Bibliográficos
Autores principales: Adachi, Tomoko, Izumi, Atsushi, Rea, Dean, Park, Sam-Yong, Tame, Jeremy R. H., Roper, David I.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2006
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225172/
https://www.ncbi.nlm.nih.gov/pubmed/17012798
http://dx.doi.org/10.1107/S1744309106035901

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225172/
https://www.ncbi.nlm.nih.gov/pubmed/17012798
http://dx.doi.org/10.1107/S1744309106035901

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