Cargando…

Expression, purification and crystallization of 2-oxo-hept-4-ene-1,7-dioate hydratase (HpcG) from Escherichia coli C

The gene encoding 2-oxo-hept-3-ene-1,7-dioic acid (OHED) hydratase (HpcG) was cloned into the high-expression plasmid pET26b and overexpressed in Escherichia coli BL21(DE3). The enzyme was purified in three steps to greater than 95% purity prior to crystallization. Crystals were obtained by the hang...

Descripción completa

Detalles Bibliográficos
Autores principales:	Adachi, Tomoko, Izumi, Atsushi, Rea, Dean, Park, Sam-Yong, Tame, Jeremy R. H., Roper, David I.
Formato:	Texto
Lenguaje:	English
Publicado:	International Union of Crystallography 2006
Materias:	Crystallization Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225172/ https://www.ncbi.nlm.nih.gov/pubmed/17012798 http://dx.doi.org/10.1107/S1744309106035901

Ejemplares similares

Expression, purification and preliminary crystallographic analysis of oligopeptidase B from Trypanosoma brucei
por: Rea, Dean, et al.
Publicado: (2006)

Expression, purification and crystallization of the SARS-CoV macro domain
por: Malet, Hélène, et al.
Publicado: (2006)

Expression, purification and preliminary crystallographic analysis of the Toxoplasma gondii enoyl reductase
por: Muench, Stephen P., et al.
Publicado: (2006)

Molecular cloning, expression, purification and crystallographic analysis of PRRSV 3CL protease
por: Tian, Xinsheng, et al.
Publicado: (2007)

Synthesis of 1-Methyl-3-oxo-7-oxabicyclo[2.2.1]hept-5-ene-2-carboxylic Acid Methyl Ester
por: Aragão, Valquiria, et al.
Publicado: (2005)

Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana
por: Bajaj, Mamta, et al.
Publicado: (2007)

Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins
por: Renzi, Fabiana, et al.
Publicado: (2006)

Overexpression, purification and crystallization of a choline-binding protein CbpI from Streptococcus pneumoniae
por: Paterson, Neil G., et al.
Publicado: (2006)

Cloning, purification and preliminary crystallographic analysis of the Bacillus subtilis GTPase YphC–GDP complex
por: Xu, Ling, et al.
Publicado: (2006)

Cloning, purification and preliminary crystallographic analysis of a putative pyridoxal kinase from Bacillus subtilis
por: Newman, Joseph A., et al.
Publicado: (2006)

Cloning, purification and crystallization of MotB, a stator component of the proton-driven bacterial flagellar motor
por: O’Neill, Jenna, et al.
Publicado: (2008)

Cloning, purification and preliminary X-ray analysis of the C-terminal domain of Helicobacter pylori MotB
por: Roujeinikova, Anna
Publicado: (2008)

Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain
por: Wei, Lei, et al.
Publicado: (2008)

Purification, crystallization and preliminary X-ray analysis of a deletion mutant of a major buckwheat allergen
por: Kezuka, Yuichiro, et al.
Publicado: (2009)

Purification, crystallization and preliminary X-ray diffraction data of UDP-galactopyranose mutase from Aspergillus fumigatus
por: Penman, George A., et al.
Publicado: (2012)

Purification, crystallization and preliminary X-ray crystallographic studies of Rv3705c from Mycobacterium tuberculosis
por: Lu, Feifei, et al.
Publicado: (2014)

Purification, crystallization and preliminary crystallographic analysis of soybean mature glycinin A1bB2
por: Prak, Krisna, et al.
Publicado: (2013)

Overexpression, purification, crystallization and data collection of Sulfolobus solfataricus Sso6206, a novel highly conserved protein
por: McEwan, Andrew R., et al.
Publicado: (2006)

Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans
por: Nishitani, Yuichi, et al.
Publicado: (2006)

Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19
por: Kirby, Jonathan M., et al.
Publicado: (2011)

Purification, crystallization and preliminary X-ray diffraction analysis of the kinase domain of human tousled-like kinase 2
por: Garrote, Ana M., et al.
Publicado: (2014)

Purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies of great cormorant (Phalacrocorax carbo) haemoglobin
por: Jagadeesan, G., et al.
Publicado: (2014)

Purification, crystallization and preliminary crystallographic characterization of the α2,6-sialyltransferase from Photobacterium sp. JT-ISH-224
por: Okino, Nozomu, et al.
Publicado: (2007)

Mammalian cell expression, purification, crystallization and microcrystal data collection of autotaxin/ENPP2, a secreted mammalian glycoprotein
por: Hausmann, Jens, et al.
Publicado: (2010)

Purification, crystallization and preliminary X-ray diffraction analysis of RafE, a sugar-binding lipoprotein from Streptococcus pneumoniae
por: Paterson, Neil G., et al.
Publicado: (2006)

Cloning, purification and preliminary crystallographic analysis of a conserved hypothetical protein, SA0961 (YlaN), from Staphylococcus aureus
por: Xu, Ling, et al.
Publicado: (2006)

Expression, purification, crystallization and preliminary crystallographic analysis of BipD, a component of the Burkholderia pseudomallei type III secretion system
por: Roversi, Pietro, et al.
Publicado: (2006)

Purification, crystallization and preliminary X-ray analysis of nonstructural protein 2 (nsp2) from avian infectious bronchitis virus
por: Yu, Kun, et al.
Publicado: (2012)

Purification, crystallization and preliminary X-ray diffraction analysis of the 23S rRNA methyltransferase RlmJ from Escherichia coli
por: Punekar, Avinash S., et al.
Publicado: (2013)

Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle
por: Deane, Janet E., et al.
Publicado: (2006)

Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231
por: Streeter, S. D., et al.
Publicado: (2009)

Diethyl 2-oxo-3-(2-oxo-2,3-dihydro-1H-indol-3-ylidene)butanedioate
por: LeninTamikovan, I. K., et al.
Publicado: (2009)

Microbial interventions are an easier alternative to engineer higher organisms
por: Keck, F. Dean, et al.
Publicado: (2020)

N-Carbamothioylamino-7-oxabicyclo[2.2.1]hept-5-ene-2,3-dicarboximide
por: Li, Jian
Publicado: (2010)

Association between serum urate level and carotid atherosclerosis: an insight from a post hoc analysis of the PRIZE randomised clinical trial
por: Tanaka, Atsushi, et al.
Publicado: (2022)

N-(4-Chlorophenyl)-7-oxabicyclo[2.2.1]hept-5-ene-2,3-dicarboximide
por: Li, Jian
Publicado: (2010)

Simultaneous purification of nitrile hydratase and amidase of Alcaligenes sp. MTCC 10674
por: Bhatia, S. K., et al.
Publicado: (2013)

Open-label randomised pragmatic trial (CONTACT) comparing naproxen and low-dose colchicine for the treatment of gout flares in primary care
por: Roddy, Edward, et al.
Publicado: (2020)

Benchmarking of the maintenance cost in HEPT Centers
por: Béjar-Alonso, Isabel, et al.
Publicado: (2002)

meso-Dimethyl 2,5-dibromohexanedioate
por: Feng, Zhi-Qiang, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_r7tmledjidft8q1he36b2eemes